Synthetic Dna

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Synthetic Dna Delivery Systems

Author : Dan Luo
ISBN : 0306477017
Genre : Science
File Size : 59.92 MB
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DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function. Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest. Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.
Category: Science

Synthetic Dna And Rna Programming

Author : Patrick O’Donoghue
ISBN : 9783039217342
Genre : Science
File Size : 37.47 MB
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Dear Colleagues, Synthetic biology is a broad and emerging discipline that capitalizes on recent advances in molecular biology, genetics, protein and RNA engineering and omics technologies. These technologies have transformed our ability to reveal the biology of the cell and the molecular basis of disease. This Special Issue on “Synthetic RNA and DNA Programming” features original research articles and reviews, highlighting novel aspects of basic molecular biology and the molecular mechanisms of disease that were uncovered by the application and development of novel synthetic biology-driven approaches.
Category: Science

Artificial Dna

Author : Yury E. Khudyakov
ISBN : 9781420040166
Genre : Science
File Size : 38.49 MB
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Combining elements of biochemistry, molecular biology, and immunology, artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis, hybridization, amplification, protein engineering, anti-sense technology, DNA vaccines, protein vaccines, recombinant antibodies, screening for genetic and pathogenic diseases, development of materials with new biochemical and structural properties, and many more. Artificial DNA: Methods and Applications introduces the concept of artificial DNA that has been rationally designed and explains how it may be exploited in order to develop products that will achieve your intended purpose. The first part of the book covers methods of oligonucleotide synthesis and direct applications of synthetic DNA. The second part describes methods of gene assembly from synthetic oligonucleotides and applications of synthetic genes. The authors also discuss the different trends and future developments within each application area . With state-of-the art research, the contributing authors describe how to engineer proteins using rational and semi-rational design to exhibit the desired traits and detail the various amplification reactions and hybridization techniques for modeling evolution and for use in basic research. The only text devoted to this subject, Artificial DNA offers a comprehensive review that allows you to understand the strategy, design, and applications of synthetic oligonucleotides.
Category: Science

Chemical Synthetic Biology

Author : Pier Luigi Luisi
ISBN : 1119990300
Genre : Science
File Size : 30.74 MB
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Chemistry plays a very important role in the emerging field of synthetic biology. In particular, chemical synthetic biology is concerned with the synthesis of chemical structures, such as proteins, that do not exist in nature. With contributions from leading international experts, Chemical Synthetic Biology shows how chemistry underpins synthetic biology. The book is an essential guide to this fascinating new field, and will find a place on the bookshelves of researchers and students working in synthetic chemistry, synthetic and molecular biology, bioengineering, systems biology, computational genomics, and bioinformatics.
Category: Science

Synthetic Biology A Primer

Author :
ISBN : 9781783268818
Genre : Science
File Size : 64.71 MB
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Synthetic Biology — A Primer (Revised Edition) presents an updated overview of the field of synthetic biology and the foundational concepts on which it is built. This revised edition includes new literature references, working and updated URL links, plus some new figures and text where progress in the field has been made. The book introduces readers to fundamental concepts in molecular biology and engineering and then explores the two major themes for synthetic biology, namely 'bottom-up' and 'top-down' engineering approaches. 'Top-down' engineering uses a conceptual framework of systematic design and engineering principles focused around the Design-Build-Test cycle and mathematical modelling. The 'bottom-up' approach involves the design and building of synthetic protocells using basic chemical and biochemical building blocks from scratch exploring the fundamental basis of living systems. Examples of cutting-edge applications designed using synthetic biology principles are presented, including: the production of novel, microbial synthesis of pharmaceuticals and fine chemicalsthe design and implementation of biosensors to detect infections and environmental waste. The book also describes the Internationally Genetically Engineered Machine (iGEM) competition, which brings together students and young researchers from around the world to carry out summer projects in synthetic biology. Finally, the primer includes a chapter on the ethical, legal and societal issues surrounding synthetic biology, illustrating the integration of social sciences into synthetic biology research. Final year undergraduates, postgraduates and established researchers interested in learning about the interdisciplinary field of synthetic biology will benefit from this up-to-date primer on synthetic biology. Contents:List of ContributorsPrefaceIntroduction to BiologyBasic Concepts in Engineering BiologyFoundational TechnologiesMinimal Cells and Synthetic LifeParts, Devices and SystemsModelling Synthetic Biology SystemsApplications of Designed Biological SystemsiGEMThe Societal Impact of Synthetic BiologyAppendices:Proforma of Common Laboratory TechniquesGlossaryIndex Readership: Students, professionals, researchers in biotechnology and bioengineering. Keywords:Synthetic Biology;Engineering Principles;Biosociety;Biological Engineering;BiotechnologyKey Features:The book is written in a way that is accessible to students and researchers from different disciplinesThe authors are part of the internationally recognised Centre for Synthetic Biology and Innovation and are among the leaders in this field
Category: Science

Advancing Development Of Synthetic Gene Regulators

Author : Anandhakumar Chandran
ISBN : 9789811065477
Genre : Science
File Size : 32.61 MB
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This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.
Category: Science

Pcr Cloning Protocols

Author : Bing-Yuan Chen
ISBN : 9781592591770
Genre : Science
File Size : 87.27 MB
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Annotation PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Category: Science

Recombinant Dna Research

Author :
ISBN : STANFORD:36105029293888
Genre : Recombinant DNA
File Size : 70.29 MB
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Category: Recombinant DNA

Dna Protein Interactions

Author : G. Geoff Kneale
ISBN : 9781592595174
Genre : Biochemistry
File Size : 86.80 MB
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The clear and easy-to-follow protocols collected here illuminate the molecular basis of protein-nucleic acid interactions. Use them successfully to reveal the location of the DNA binding site, the strength and specificity of a binding, the identities of individual groups on the actual bases involved in binding, and the specific amino acid residues of the protein that interact with the DNA. Some of the techniques can even be used to identify previously unknown DNA binding proteins from crude cell extracts, thus empowering you to make groundbreaking advances in your work.
Category: Biochemistry

Synthetic Biology

Author : National Academies Keck Futures Initiative
ISBN : 9780309149426
Genre : Science
File Size : 36.63 MB
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Synthetic biology is an innovative and growing field that unites engineering and biology. It builds on the powerful research that came about as a result of a recombinant DNA technology and genome sequencing. By definition, synthetic biology is an interdisciplinary enterprise comprising biologists of many specialties, engineers, physicists, computer scientists and others. It promises a fundamentally deeper understanding of how living systems work and the capacity to recreate them for medicine, public health and the environment, including renewable energy. NAKFI Synthetic Biology: Building a Nation's Inspiration discusses new foundational technologies and tools required to make biology easier to engineer, considers ethical issues unique to synthetic biology, explores how synthetic biology can lead to an understanding of the principles underlying natural genetic circuits and debates how synthetic biology can be used to answer fundamental biological questions.
Category: Science

Analytics Of Protein Dna Interactions

Author : Claude Backendorf
ISBN : 9783540481478
Genre : Medical
File Size : 27.88 MB
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Advances in Biochemical Engineering/Biotechnology reviews actual trends in modern biotechnology. Its aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required for chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. They give the state-of-the-art of a topic in a comprehensive way thus being a valuable source for the next 3 - 5 years. It also discusses new discoveries and applications. Special volumes are edited by well known guest editors who invite reputed authors for the review articles in their volumes. Readership: research scientists at universities or in industry, graduate students
Category: Medical

Synthesis And Applications Of Dna And Rna

Author : Saran Narang
ISBN : 9780323158886
Genre : Science
File Size : 49.72 MB
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Synthesis and Applications of DNA and RNA discusses the significant contributions in the development of synthetic routes to DNA and RNA. This book contains nine chapters that describe the complexities in the chemistry and biology of DNA and RNA. After briefly dealing with the various stages of development in the chemical synthesis of polynucleotides, this book goes on presenting the DNA synthesis on solid supports and through the phosphoramidite method on silica supports. The discussions then shift to the chemical-enzymatic synthesis of expressed genes; the biochemical aspects of chemical syntheses of oligoribonucleotides; and the methods of rapid DNA and RNA sequence analysis. A chapter specifically tackles the protocols of DNA synthesis using double-stranded plasmid DNA as a template. The final chapter deals with the use of oligonucleotides for the identification and isolation of specific gene sequences. This chapter also covers the use oligonucleotides in the detection of human genetic diseases. Biologists, geneticists, and researchers interested in DNA and RNA synthesis will find this work invaluable.
Category: Science

Dna Repair

Author :
ISBN : 0080463444
Genre : Science
File Size : 39.40 MB
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DNA Repair, Part A provides detailed coverage of modern methods for molecular analysis of enzymes and enzyme systems that function in the maintenance of genome integrity. Coverage areas include base excision repair, nucleotide excision repair, translesion DNA polymerases, mismatch repair, genetic recombination, and double strand break repair. A laboratory standard for more than 40 years Over 400 volumes strong Also available on ScienceDirect Part A of a 2-part series
Category: Science

Life At The Speed Of Light

Author : J. Craig Venter
ISBN : 9781101638026
Genre : Science
File Size : 65.68 MB
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“Venter instills awe for biology as it is, and as it might become in our hands.” —Publishers Weekly On May 20, 2010, headlines around the world announced one of the most extraordinary accomplishments in modern science: the creation of the world’s first synthetic lifeform. In Life at the Speed of Light, scientist J. Craig Venter, best known for sequencing the human genome, shares the dramatic account of how he led a team of researchers in this pioneering effort in synthetic genomics—and how that work will have a profound impact on our existence in the years to come. This is a fascinating and authoritative study that provides readers an opportunity to ponder afresh the age-old question “What is life?” at the dawn of a new era of biological engineering.
Category: Science

Dna Computing

Author : Ashish Goel
ISBN : 9783642030758
Genre : Computers
File Size : 75.60 MB
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This book constitutes the thoroughly refereed post-conference proceedings of the 14th International Meeting on DNA Computing, DNA 14, held in Prague, Czech Republic, in June 2008. The 15 revised full papers presented were carefully reviewed and selected from 59 submissions. Their topics include theoretical models of biomolecular computing, demonstrations of biomolecular computing processes, self-assembly systems, DNA nanostructures and nanomachines, biotechnological and other applications of DNA computing, and other related themes.
Category: Computers

Biometric Inverse Problems

Author : Svetlana N. Yanushkevich
ISBN : 1420037978
Genre : Technology & Engineering
File Size : 52.83 MB
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Traditional methods of biometric analysis are unable to overcome the limitations of existing approaches, mainly due to the lack of standards for input data, privacy concerns involving use and storage of actual biometric data, and unacceptable accuracy. Exploring solutions to inverse problems in biometrics transcends such limits and allows rich analysis of biometric information and systems for improved performance and testing. Although some particular inverse problems appear in the literature, until now there has been no comprehensive reference for these problems. Biometric Inverse Problems provides the first comprehensive treatment of biometric data synthesis and modeling. This groundbreaking reference comprises eight self-contained chapters that cover the principles of biometric inverse problems; basics of data structure design; new automatic synthetic signature, fingerprint, and iris design; synthetic faces and DNA; and new tools for biometrics based on Voronoi diagrams. Based on the authors' vast experience in the field, the book authoritatively examines new approaches and methodologies in both direct and inverse biometrics, providing invaluable analytical and benchmarking tools. The authors include case studies, examples, and implementation codes for practical illustration of the methods. Loaded with approximately 200 figures, 60 problems, 50 MATLAB® code fragments, and 200 examples, Biometric Inverse Problems sets the standard for innovation and authority in biometric data synthesis, modeling, and analysis.
Category: Technology & Engineering

Dna Technology

Author : I. Edward Alcamo
ISBN : 0120489201
Genre : Science
File Size : 84.47 MB
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Gives the educated layperson a survey of DNA by presenting a brief history of genetics, an outline of techniques, and indications of breakthroughs in cloning and other DNA advances. This book helps students, business people, lawyers, and jurists gain confidence in their ability to understand and appreciate DNA technology and human genetics.
Category: Science

Protein Engineering Handbook

Author : Stefan Lutz
ISBN : 9783527667017
Genre : Science
File Size : 68.35 MB
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Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.
Category: Science