Rice Protocols

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Rice Protocols

Author : Yinong Yang
ISBN : 1627031936
Genre : Science
File Size : 32.51 MB
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With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
Category: Science

Pre Field Screening Protocols For Heat Tolerant Mutants In Rice

Author : Fatma Sarsu
ISBN : 9783319773384
Genre : Science
File Size : 51.71 MB
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This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.
Category: Science

Protocols For Pre Field Screening Of Mutants For Salt Tolerance In Rice Wheat And Barley

Author : Souleymane Bado
ISBN : 9783319265902
Genre : Science
File Size : 39.60 MB
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This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and barley seedlings, which uses hydroponics. The protocols are devised for use by plant breeders and can be easily accommodated into breeding practice.
Category: Science

Agrobacterium Protocols

Author : Kan Wang
ISBN : 9781597451307
Genre : Science
File Size : 47.36 MB
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"A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls."--Publisher's website.
Category: Science

Plant Cell Culture Protocols

Author : Robert D. Hall
ISBN : 9781592595839
Genre : Science
File Size : 67.37 MB
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In Plant Cell Culture Protocols, Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. In addition, a number of specialized protocols have been included to illustrate the diversity of the techniques available and their widespread applicability. Plant Cell Culture Protocols is aimed at scientists involved in all aspects of plant biotechnological research, as well as those working in other areas of agriculture and horticulture who are interested in expanding their technical repertoire to include in vitro methodology. Its state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.
Category: Science

Plant Virology Protocols

Author : Gary D. Foster
ISBN : 9781592595662
Genre : Science
File Size : 83.40 MB
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This text elucidates the latest techniques in plant virology for the isolation of plant viruses, for RNA extraction, and for the localization and cloning of coat protein genes, among others.
Category: Science

Rice Genome Engineering And Gene Editing

Author : Anindya Bandyopadhyay
ISBN : 1071610678
Genre :
File Size : 40.18 MB
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This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.

Rice Grain Quality

Author : Nese Sreenivasulu
ISBN : 1493994107
Genre : Science
File Size : 67.70 MB
Format : PDF
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This volume explores the latest advances in high-throughput phenotyping to screen milling quality, cooking and nutritional quality in breeding material, and simplifying grain quality screening in germplasm. The chapters in this book cover topics such as seed development stages; panicle architectural traits to understand yield components; starch structure and protein properties; and genome editing technologies to reveal grain quality and nutritional traits. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rice Grain Quality: Methods and Protocols is a valuable resource for all researchers interested in expanding their knowledge in this evolving field.
Category: Science

Biological Control Of Rice Diseases

Author : Samuel S. Gnanamanickam
ISBN : 9789048124657
Genre : Science
File Size : 40.94 MB
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There is suf?cient need to document all the available data on biological control of rice diseases in a small volume. Part of this need rests on the global importance of rice to human life. In the ?rst chapter, I have tried to show that rice is indeed life for most people in Asia and shortages in production and availability can lead to a food crisis. While rice is cultivated in most continents, biological disease management attains special relevance to rice farmers of Africa, Asia, and also perhaps, Latin America. These farmers are resource-poor and might not be able to afford the cost of expensive chemical treatments to control devastating rice pathogens such as Magnaporthe oryzae (blast), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), Rhizoctonia solani (sheath blight) and the virus, rice tungro disease. In an earlier volume that I developed under the title, Biological Control of Crop Diseases (Dekker/CRC Publishers, 2002), I included transgenic crops generated for the management of plant pathogens as biological control under the umbrella of a broad de?nition. Dr Jim Cook who wrote the Foreword for the volume lauded the inclusion of transgenic crops and induced systemic resistance (ISR) as a positive trend toward acceptance of host plant resistance as part of biocontrol. I continue to subscribe to this view.
Category: Science

Doubled Haploid Production In Crop Plants

Author : M. Maluszynski
ISBN : 1402015445
Genre : Nature
File Size : 47.94 MB
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Includes various protocols and approaches of DH production proven for different germplasm of the same species.
Category: Nature


Author : Francesca Bray
ISBN : 9781107044395
Genre : Business & Economics
File Size : 45.75 MB
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Rice is a first step toward a history of rice and its place in capitalism from global and comparative perspectives.
Category: Business & Economics

Rice Genetics Iii

Author : Gurdev S. Khush
ISBN : 9789712200878
Genre : Electronic books
File Size : 63.60 MB
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Plenary session papers; I: Varietal differentiation and evolution; II: Genetics of morphological and physiological traits; III: Genetics of disease resistance; IV: Cytogenetics; V: Tissue and cell culture; VI: Molecular mapping of genes; VII: Map-based gene cloning; VIII: Molecular genetics of cytoplasmic male sterility; IX: Transformation; X: Gene isolation, characterization, and expression; XI: Genetic diversity in pathogen populations; XII: Rice research priorities.
Category: Electronic books

Rice Functional Genomics

Author : Narayana M. Upadhyaya
ISBN : 9780387489148
Genre : Science
File Size : 37.11 MB
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Rice has been chosen as a model cereal for functional genomics because of its well understood genetics. In addition, there is great similarities among all the cereals and grasses: any understanding of rice genes is directly applicable to the genes of other cereals. This book details rice functional genomics. It takes a multi-pronged genome-wide approach using structural similarities, expression profiles, and mutant phenotypes. Coverage includes the current status of genome sequencing and annotation, various tools, and resources being developed worldwide.
Category: Science

Plant Pathogen Interactions

Author : Pamela C. Ronald
ISBN : 9781592599660
Genre : Medical
File Size : 86.85 MB
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Plant-Pathogen Interactions gathers together some of the key methods used in studies of this field and includes chapters describing how this knolwedge is being used to develop new strategies for disease control.
Category: Medical

The Quest For Nitrogen Fixation In Rice

Author : Jagdish Kumar Ladha
ISBN : 9789712201127
Genre : Bacteria, Nitrifying
File Size : 58.92 MB
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This book features the latest research advances made in developing nitrogen-fixing rice.
Category: Bacteria, Nitrifying

Electroporation Protocols For Microorganisms

Author : Jac A. Nickoloff
ISBN : 9781592595341
Genre : Science
File Size : 34.62 MB
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Electroporation Protocols for Microorganisms is the first complete guide to the electroporation of nearly all microorganisms of importance used in biological and biomedical research. It includes reproducible protocols for diverse bacterial, fungal, and protist species - many of which are important in human disease - as well as literature references to electroporation protocols for related species. The contributors also discuss electroporation theory and instrumentation, making it possible to develop new protocols or modify existing ones, and they provide extensive details about culturing and storing many species in a manner designed to optimize electroporation efficiency. Electroporation Protocols for Microorganisms is an indispensable resource for molecular geneticists working directly with microorganisms and for those who employ microorganisms to prepare materials for later introduction into higher organisms, such as plants and animals. Two companion volumes will follow: Plant Cell Electroporation and Electrofusion Protocols and Animal Cell Electroporation and Electrofusion Protocols.
Category: Science

Aromatic Rices

Author :
ISBN : 9788120414204
Genre : Aromatic plants
File Size : 65.15 MB
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Category: Aromatic plants

Plant Bioinformatics

Author : David Edwards
ISBN : 9781588296535
Genre : Science
File Size : 60.16 MB
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This book aims to assist research scientists in choosing the most applicable database or bioinformatics tools to aid and promote their research in plant biotechnology. Chapters include practical examples and highlight common problems encountered in bioinformatics analysis. Further chapters are aimed at researchers developing bioinformatics databases and tools, detailing commonly applied database formats and biology-focused scripting languages.
Category: Science