Plant Embryo Culture

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Plant Embryo Culture

Author : Trevor A. Thorpe
ISBN : 1493957899
Genre : Science
File Size : 73.99 MB
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A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular BiologyTM series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.
Category: Science

Introduction To Plant Tissue Culture

Author : M. K. Razdan
ISBN : 1578082374
Genre : Science
File Size : 83.75 MB
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Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.
Category: Science

Introduction To Plant Biotechnology

Author : H. S. Chawla
ISBN : 1578082285
Genre : Technology & Engineering
File Size : 56.84 MB
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Plant biotechnology has created unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. This text on the subject is aimed at students.
Category: Technology & Engineering

In Vitro Culture Of Higher Plants

Author : R.L.M Pierik
ISBN : 9789401157506
Genre : Science
File Size : 25.1 MB
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In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.
Category: Science

Plant Tissue Culture

Author : Timir Baran Jha
ISBN : 8173714886
Genre : Plant tissue culture
File Size : 21.97 MB
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Plant Tissue Culture In One Form Or Another Has Become One Of The Most Promising Branches Of Plant Science. Arising From The Totipotency Of Plant Cells, It Now Occupies A Key Position In Plant Breeding, Plant Propagation And Plant Biotechnology.Plant Tissue Culture - Basic And Applied Brings To The Student Accessible, Up-To-Date Information On This Subject. Basic Knowledge Of Tissue Culture Methods Such As Isolation Of Suitable Tissues From The Mother Plant, Maintenance Of The Tissues Under In Vitro Condition In An Undifferentiated Or De-Differentiated Stage, Methods Of Genetic Engineering And Gene Transfer, Chromosomal Studies And The Handling Of In Vitro Micro Plants Are Described In Detail In This Book. Similarly, Application Aspects Of Micropropagation, Haploid Cell Culture, Protoplast Culture, Embryo Culture, Somatic Embryogenesis And Artificial Seeds Are Also Discussed.
Category: Plant tissue culture

Plant Tissue Culture An Introductory Text

Author : Sant Saran Bhojwani
ISBN : 9788132210269
Genre : Science
File Size : 22.7 MB
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Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.
Category: Science

Plant Tissue Culture

Author : Roberta H. Smith
ISBN : 9780124159204
Genre : Science
File Size : 56.14 MB
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Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
Category: Science

Plant Cell Culture

Author : David E. Evans
ISBN : 185996320X
Genre : Science
File Size : 84.52 MB
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Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf.
Category: Science

Plant Cell Culture Protocols

Author : Victor M. Loyola-Vargas
ISBN : 9781592599592
Genre : Science
File Size : 82.92 MB
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This second edition of Plant Cell Protocols follows a similar vein as its successful predecessor in providing an updated step=by-step guide to the most common, and applicable techniques and methods for plant tissue and cell culture. A total of 30 chapters, divided into 6 major sections have been included. Topics selected cover from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation, passing through the laborious process of protoplast isolation and culture. The protocols are currently used in the research programs of the authors, or represent important parts of business projects aimed at the generation of improved plant materials.
Category: Science

Plant Tissue Culture

Author : B. N. Sathyanarayana
ISBN : 9788189866112
Genre : Botany, Experimental
File Size : 62.39 MB
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Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.
Category: Botany, Experimental

Plant Tissue Culture Manual

Author : K. Lindsey
ISBN : 0792320271
Genre : Plant tissue culture
File Size : 40.37 MB
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The third supplement to the manual expands the coverage of existing sections and introduces the new section Mutant Selection. Future supplements will continue to update existing sections and will also introduce new sections on Secondary Metabolites and Tissue Culture Techniques for Fundamental Studies, maintaining the manual as a practical and up-to-date source of lab bench procedures.
Category: Plant tissue culture

Plant Tissue Culture

Author : Robert H. Smith
ISBN : 9780323160476
Genre : Science
File Size : 83.17 MB
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Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Category: Science

Plant Tissue Culture

Author : S.S. Bhojwani
ISBN : 9780444598479
Genre : Science
File Size : 34.14 MB
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During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly. This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.
Category: Science

Plant Tissue Culture Theory And Practice

Author : S.S. Bhojwani
ISBN : 0080539092
Genre : Science
File Size : 70.68 MB
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Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each chapter has been thoroughly revised and, as before, is written in lucid language, includes relevant media protocols, and is profusely illustrated with self-explanatory diagrams and original photographs. This book includes three new chapters: "Variant selection", "Genetic Engineering" and "Production of Industrial Compounds" and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature. This updated version proves to be an excellent text for undergraduate, postgraduate students and teachers in various fields of plant sciences and a useful reference book for those interested in the application of any aspect of this aseptic technology.
Category: Science

Morphogenesis In Plant Tissue Cultures

Author : Woong-Young Soh
ISBN : 0792356829
Genre : Science
File Size : 90.44 MB
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This book presents a detailed analysis of up-to-date literature on in vitro morphogenesis at cell, tissue, organ, and whole plant levels. Its driving force is the substantial advances made in the field of morphogenesis in tissue cultures during the last 25 years.
Category: Science

Tissue Culture

Author : Henry Gilbert
ISBN : 0788121804
Genre :
File Size : 41.29 MB
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A collection of 273 citations in English on the topic of tissue culture in plants, including embryos, pollen, cell suspension, buds, in vitro culture, etc. Most citations have abstracts. Author and subject index.

Breeding Field Crops

Author : John M. Poehlman
ISBN : 9789401572712
Genre : Juvenile Nonfiction
File Size : 72.70 MB
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While preparing the first edition of this textbook I attended an extension short course on writing agricultural publications. The message I remember was "select your audience and write to it. " There has never been any doubt about the audience for which this textbook was written, the introductory course in crop breeding. In addition, it has become a widely used reference for the graduate plant-breeding student and the practicing plant breeder. In its prepa ration, particular attention has been given to advances in plant-breeding theo ry and their utility in plant-breeding practice. The blend of the theoretical with the practical has set this book apart from other plant-breeding textbooks. The basic structure and the objectives of the earlier editions remain un changed. These objectives are (1) to review essential features of plant re production, Mendelian genetic principles, and related genetic developments applicable in plant-breeding practice; (2) to describe and evaluate established and new plant-breeding procedures and techniques, and (3) to discuss plant breeding objectives with emphasis on the importance of proper choice of objec tive for achieving success in variety development. Because plant-breeding activities are normally organized around specific crops, there are chapters describing breeding procedures and objectives for the major crop plants; the crops were chosen for their economic importance or diversity in breeding sys tems. These chapters provide a broad overview of the kinds of problems with which the breeder must cope.
Category: Juvenile Nonfiction

Plant Tissue Culture And Moelcular Markers

Author : Ashwani Kumar
ISBN : 9788189866105
Genre : Agricultural productivity
File Size : 35.21 MB
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Besides, recently molecular biology has assumed great importance with respect to plant biotechnology. The present book amalgamates all three aspects into one, practical applications of various techniques being the need of the hour. It discusses micropropagation studies on several crop plants, molecular basis of understanding various life processes including molecular basis of somatic embryogenesis and other physiological and biochemical processes having significant biotechnological applications. It also includes in vitro studies of some important plants like Aloe vera, Simmondsia chinensis, Anacyclus pyrethrum and Crataeva nurvala, Arachis hypogaea L., Phoenix dactylifera, Dendrocalamus asper, Asparagus adescendens Roxb., natural products of plant origin with their therapeutic potential and biotechnological production, genome analysis of crop plants with future applications in biotechnology etc.
Category: Agricultural productivity

Plant Biotechnology And Molecular Markers

Author : S. Srivastava
ISBN : 9781402032134
Genre : Science
File Size : 59.62 MB
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The genesis of the volume, Plant Biotechnology and Molecular Markers, has been the occasion of the retirement of Professor Sant Saran Bhojwani from the Department of Botany, University of Delhi. For Professor Bhojwani, retirement only means relinquishing the chair as being a researcher and a teacher which has always been a way of life to him. Professor Bhojwani has been an ardent practitioner of modern plant biology and areas like Plant Biotechnology and Molecular Breeding have been close to his heart. The book contains original as well as review articles contributed by his admirers and associates who are experts in their area of research. While planning this contributory book our endeavour has been to incorporate articles that cover the entire gamut of Plant Biotechnology, and also applications of Molecular Markers. Besides articles on in vitro fertilization and micropropagation, there are articles on forest tree improvement through genetic engineering. Considering the importance of conservation of our precious natural wealth, one article deals with cryopreservation of plant material. Chapter on molecular marker considers DNA indexing as markers of clonal fidelity of in vitro regenerated plants and prevention against bio-piracy. A couple of write-ups also cover stage-specific gene markers, DNA polymorphism and genetic engineering, including raising of stress tolerant plants to sustain productivity and help in reclamation of degraded land.
Category: Science

Plant Tissue Culture Development And Biotechnology

Author : Robert N. Trigiano
ISBN : 9781439896143
Genre : Medical
File Size : 80.71 MB
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Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
Category: Medical