Nitroxide Spin Labels

Download Nitroxide Spin Labels ebook PDF or Read Online books in PDF, EPUB, and Mobi Format. Click Download or Read Online button to Nitroxide Spin Labels book pdf for free now.

Nitroxide Spin Labels

Author : Nickolai Kocherginsky
ISBN : 084934204X
Genre : Science
File Size : 78.82 MB
Format : PDF, Kindle
Download : 111
Read : 1149

Extending the use of nitroxides as probes into more complex systems such as viable cells and whole animals requires greater knowledge of their interactions in functioning biological systems and appropriate model systems. Nitroxide Spin Labels: Reactions in Biology and Chemistry presents information essential for research in this area. Nitroxide research offers the promise of important new ways of measuring metabolism and will be useful in the diagnosis of important disease categories such as cancer, inflammation, and ischemia.
Category: Science

Site Directed Nitroxide Spin Labeling Of Oligonucleotides For Nmr Andepr Studies

Author :
ISBN : OCLC:1051883036
Genre :
File Size : 25.46 MB
Format : PDF, ePub, Mobi
Download : 352
Read : 201

Abstract: Nitroxide labels are useful probes of biomolecule structure that can be detected by NMR and EPR spectroscopy. Although many methods exist for labeling oligonucleotides with nitroxides, most require reagents that are expensive or laborious to prepare. A simpler approach is described herein using commercially available phosphorothioate oligonucleotides and 2-(3-iodoacetamidomethyl)-PROXYL. We describe semi-optimized conditions for labeling DNA and RNA oligonucleotides and methodology for purifying and identifying these reagents by MALDI-MS. The nitroxide label showed some propensity to hydrolyze at high temperature and over prolonged periods at room temperature. Nitroxide-labeled DNA oligonucleotides gave characteristic EPR spectra and caused the disappearance of bound protein signals in 15 N HSQC spectra consistent with the paramagnetic relaxation enhancement (PRE) effect. Graphical abstract:
Category:

A Cytidine Phosphoramidite With Protected Nitroxide Spin Label Synthesis Of A Full Length Tar Rna And Investigation By In Line Probing And Epr Spectroscopy

Author :
ISBN : OCLC:1052005692
Genre :
File Size : 64.34 MB
Format : PDF, ePub, Mobi
Download : 856
Read : 642

Abstract: EPR studies on RNA are complicated by three major obstacles related to the chemical nature of nitroxide spin labels: Decomposition while oligonucleotides are chemically synthesized, further decay during enzymatic strand ligation, and undetected changes in conformational equilibria due to the steric demand of the label. Herein possible solutions for all three problems are presented: A 2‐nitrobenzyloxymethyl protective group for nitroxides that is stable under all conditions of chemical RNA synthesis and can be removed photochemically. By careful selection of ligation sites and splint oligonucleotides, high yields were achieved in the assembly of a full‐length HIV‐1 TAR RNA labeled with two protected nitroxide groups. PELDOR measurements on spin‐labeled TAR in the absence and presence of arginine amide indicated arrest of interhelical motions on ligand binding. Finally, even minor changes in conformation due to the presence of spin labels are detected with high sensitivity by in‐line probing. Abstract : Spin labels stabilized : Nitroxides are important spin labels for EPR studies on biopolymers, but sample preparation can be severely restricted by their limited chemical stability. The 2‐nitrobenzyloxymethyl group protects the TEMPO spin label against all critical conditions of chemical RNA synthesis and enzymatic ligation (see figure). After photochemical deprotection, the nitroxide radical is recovered by spontaneous air oxidation. Assembly of a spin‐labeled 59mer RNA is shown as an example.
Category:

Spin Labeling

Author : Lawrence J. Berliner
ISBN : 9781483216416
Genre : Science
File Size : 70.36 MB
Format : PDF, ePub
Download : 569
Read : 1010

Spin Labeling II: Theory and Applications covers spin-labeled biopolymers and its importance to both macromolecular modeling as well as some industrially based problems. The book discusses the methods of saturation-transfer spectroscopy; the spin-probe-spin-label method; and new aspects of nitroxide chemistry. The text also describes the spin-labeled synthetic polymers; the impact that the technique of spin labeling has on pharmacology and on biomedicine; as well as the applications of spin labeling to nucleic acids. Chemists, pharmacologists, biochemists, and people working in the field of biological magnetic resonance will find the book invaluable.
Category: Science

Structural Information From Spin Labels And Intrinsic Paramagnetic Centres In The Biosciences

Author : Christiane R. Timmel
ISBN : 9783642391255
Genre : Science
File Size : 86.11 MB
Format : PDF, ePub
Download : 377
Read : 491

Pulse Dipolar Electron Spin Resonance: Distance Measurements by Peter P. Borbat, Jack H. Freed.Interpretation of Dipolar EPR Data in Terms of Protein Structure, by Gunnar Jeschke.Site-Directed Nitroxide Spin Labeling of Biopolymers, by Sandip A. Shelke and Snorri Th. Sigurdsson. Metal-Based Spin Labeling for Distance Determination, by Daniella Goldfarb. Structural Information from Spin-Labelled Membrane-Bound Proteins, by Johann P. KLare, Heinz-Jürgen Steinhoff. Structural Information from Oligonucleotides, by Richard Ward and Olav Schiemann. Orientation selective DEER using rigid spin labels, cofactors, metals, and clusters, by Claudia E. Tait, Alice M. Bowen, Christiane R. Timmel, Jeffrey Harmer
Category: Science

Spin Labeling

Author : Lawrence Berliner
ISBN : 9780306456442
Genre : Science
File Size : 44.21 MB
Format : PDF, Kindle
Download : 434
Read : 789

We present here the second issue devoted entirely to the spin-labeling technique as part of Biological Magnetic Resonance. Volume 14 commemorates a modifi- tion in our editorial policy with the retirement of my esteemed coeditor, Jacques Reuben. From thisjuncture into the future, each issue will focus on some special topic in magnetic resonance. Each volume will be organized in most cases by guest editors, for example forthcoming issues will address the following topics: in vivo magnetic resonance (P. Robitaille and L. J. Berliner, eds. ) Modern techniques in proton NMR ofproteins (R. Krishna and L. J. Berliner, eds. ) Instrumental techniques of EPR (C. Bender and L. J. Berliner, eds. ) Thecurrent volume, Spin Labeling: The NextMillennium, presents an excellent collection of techniques and applications that evolved during the past decade since the last volume, volume 8 (1989). Someobvious omissions, such as multiquantum EPR and very high-frequency FT-ESR were unfortunately not possible for this volume. Perhaps they will appear in Spin Labeling: 2001. Lastly it is a pleasure to honor two scientists whose contributions were both pioneering and pivotal to the spin label technique: Professor Eduard G. Rozantsev (Moscow), whose synthetic feats in nitroxyl chemistry set the broad stage for a versatile catalog of labels; and Professor Harden M. McConnell, last year's Int- national ESR (EPR) Society Gold Medalist, who conceived and developed the spin label technique to address many biological problems (proteins, enzymes, m- branes, cells, immune response, etc. ). Lawrence J.
Category: Science

Bioactive Spin Labels

Author : Renat I. Zhdanov
ISBN : 9783642487248
Genre : Science
File Size : 56.14 MB
Format : PDF, Docs
Download : 123
Read : 207

Bioactive Spin Labels is a collection of contributions by leading experts on nitroxyl radicals and their application in biomedicine. These compounds are used as side specific contrast agents for in-vivo EPR techniques. Their potential as drugs is investigated. Bioactive Spin Labels is a must for researchers in bioorganic chemistry, pharmacology and biomedicine.
Category: Science

Electron Paramagnetic Resonance Investigations Of Biological Systems By Using Spin Labels Spin Probes And Intrinsic Metal Ions

Author :
ISBN : 9780128028476
Genre : Medical
File Size : 80.29 MB
Format : PDF, ePub, Mobi
Download : 740
Read : 794

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function. Timely contribution that describes a rapidly changing field Leading researchers in the field Broad coverage: Instrumentation, basic theory, data analysis, and applications
Category: Medical

Electron Paramagnetic Resonance Investigations Of Biological Systems By Using Spin Labels Spin Probes And Intrinsic Metal Ions

Author :
ISBN : 9780128028469
Genre : Medical
File Size : 49.65 MB
Format : PDF, Mobi
Download : 953
Read : 642

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function. Timely contribution that describes a rapidly changing field Leading researchers in the field Broad coverage: Instrumentation, basic theory, data analysis, and applications
Category: Medical

Distance Measurements Using Pulsed Epr

Author : Gunnar Widtfeldt Reginsson
ISBN : OCLC:857996516
Genre : Biomolecules
File Size : 66.75 MB
Format : PDF, ePub, Mobi
Download : 395
Read : 874

The function of biomacromolecules is controlled by their structure and conformational flexibility. Investigating the structure of biologically important macromolecules can, therefore, yield information that could explain their complex biological function. In addition to X ray crystallography and nuclear magnetic resonance (NMR) methods, pulsed electron paramagnetic resonance (EPR) methods, in particular the pulsed electron electron double resonance (PELDOR) technique has, during the last decade, become a valuable tool for structural determination of macromolecules. Long range distance constraints obtained from pulsed EPR measurements, make it possible to carry out structural refinements on structures from NMR and X ray methods. In addition, EPR yields distance distributions that give information about structural flexibility. The use of EPR for structural studies of biomacromolecules requires in most cases site specific incorporation of paramagnetic centres known as spin labelling. To date, spin labelling nucleic acids has required complex spin labelling chemistry. The first application of a site directed and noncovalent spin labelling method for distance measurements on DNA is described. It is demonstrated that noncovalent spin labelling with a rigid spin label can afford detailed information on internal DNA dynamics using PELDOR. Furthermore, it is shown that noncovalent spin labelling can be used to study DNA protein complexes. PELDOR can also yield information about spin label orientation. Therefore, spin labels with limited flexibility can be used to measure the relative orientation of the spin labelled sites. Although information on orientation can be obtained from 9.7 GHz PELDOR measurements in selected applications, measurements at 97 GHz or higher, increases orientation selection. It is shown that PELDOR measurements on semi rigid and rigid nitroxide biradicals using a home built high power 97 GHz EPR spectrometer (Hiper) and model based simulations yield quantitative information on spin label orientations and dynamics. The most widely used spin labels for EPR studies on biomacromolecules are the aminoxyl (nitroxide) radicals. The major drawbacks of nitroxide spin labels include low sensitivity for distance measurements, fast spin spin relaxation in solution and limited stability in reducing environments. Carbon centered triarylmethyl (trityl) radicals have properties that could eliminate some of the limitations of nitroxide spin labels. To evaluate the use of trityl spin labels for nanometer distance measurements, models systems with trityl and nitroxide spin labels were measured using PELDOR and Double Quantum Coherence (DQC). This study shows that trityl spin labels yield reliable information on interlabel distances and dynamics, establishing the trityl radical as a viable spin label for structural studies on biomacromolecules.
Category: Biomolecules

Electron Paramagnetic Resonance

Author : Victor Chechik
ISBN : 9781788013727
Genre : Science
File Size : 31.25 MB
Format : PDF
Download : 756
Read : 680

Electron Paramagnetic Resonance (EPR) applications remain very significant in modern analytical science and this volume compiles critical coverage of developments in the recent literature by a handpicked group of researchers at the cutting-edge of the field. The topics covered in this volume describe contrasting types of EPR application, including light induced hyperpolarization and disordered proteins to spin labels and nanomaterials. Providing a snap shot of the area, this book is a useful addition to any library supporting this research.
Category: Science

Spin Labeling Ii

Author : Lawrence J. Berliner
ISBN : UOM:39015007488789
Genre : Electron paramagnetic resonance
File Size : 60.97 MB
Format : PDF, ePub, Mobi
Download : 980
Read : 789

Spin Labeling II: Theory and Applications covers spin-labeled biopolymers and its importance to both macromolecular modeling as well as some industrially based problems. The book discusses the methods of saturation-transfer spectroscopy; the spin-probe-spin-label method; and new aspects of nitroxide chemistry. The text also describes the spin-labeled synthetic polymers; the impact that the technique of spin labeling has on pharmacology and on biomedicine; as well as the applications of spin labeling to nucleic acids. Chemists, pharmacologists, biochemists, and people working in the field of bi ...
Category: Electron paramagnetic resonance

Riboswitch Discovery Structure And Function

Author :
ISBN : 9780128013359
Genre : Science
File Size : 73.51 MB
Format : PDF, ePub, Mobi
Download : 814
Read : 674

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, and ligand interactions and thermodynamics. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in riboswitch discovery, structure and function Contains sections on such topics as riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, ligand interactions and thermodynamics
Category: Science

Esr Spectroscopy In Membrane Biophysics

Author : Marcus A. Hemminga
ISBN : 9780387250663
Genre : Medical
File Size : 78.94 MB
Format : PDF, Mobi
Download : 395
Read : 553

Spectroscopic methods are not only important as an analytical tool, they also provide information about fundamental physical and chemical properties of molecules, the molecular and electronic structure, and the dynamic behaviour of molecules. Starting from a comprehensive quantum mechanical description, ESR Spectroscopy in Membrane Biophysics introduces the optical (IR, Raman, UV/Vis, CD, fluorescence and laser spectroscopy) and magnetic resonance (1D and 2D-NMR, ESR) techniques. ESR Spectroscopy in Membrane Biophysics is a timely review of the increasing interest in using spin-label ESR as an alternative structural technique for NMR or X-ray diffraction. It is aimed at training an audience to learn ESR spectroscopy to determine membrane protein structures, conformational dynamics and protein-lipid interaction.
Category: Medical

Electron Spin Resonance

Author : R. O. C. Norman
ISBN : 9780851867618
Genre : Reference
File Size : 80.79 MB
Format : PDF, ePub, Mobi
Download : 679
Read : 1098

Reflecting the growing volume of published work in this field, researchers will find this book an invaluable source of information on current methods and applications.
Category: Reference

Very High Frequency Vhf Esr Epr

Author : Oleg Grinberg
ISBN : 0306483165
Genre : Medical
File Size : 76.50 MB
Format : PDF, ePub, Docs
Download : 342
Read : 382

The field of Very High Frequency EPR (VHF EPR) or sometimes called Very High Field EPR (conveniently, also abbreviated as VHF EPR) has blossomed during the past decade, especially after the original pioneering work of Ya. S. Lebedev and his group at the Institute of Chemical Physics, Russian Academy of Sciences in Moscow. Although Lebedev suffered heavily under the economic constraints of the communist Soviet Union and then succumbed to cancer at the peak of his scientific career, his groundbreaking work from the 1970's is still considered today to be the 'gold standard' by researchers practicing EPR at high magnetic fields. A stimulus for the production of this book is the legacy of Yakov Levedev in his students now residing in academic positions in the US and elsewhere. The aim of this book is to highlight the state of this growing field. This is an attempt to cover the full scope of VHF EPR in a single volume. The idea for this volume came to the editors at the 2001 Rocky Mountain Analytical Conference during the 24th International EPR Symposium chaired by Sandra and Gareth Eaton. VHF EPR was presented as an independent research field at a workshop organized by LC BruneI and supported by the National High Magnetic Field Laboratory, a National Science Foundation funded facility at Florida State University.
Category: Medical

Nitroxides

Author : Gertz I. Likhtenshtein
ISBN : 9783030348229
Genre : Science
File Size : 83.34 MB
Format : PDF, ePub, Docs
Download : 896
Read : 938

Written by a pioneer in the development of spin labeling in biophysics, this expert book covers the fundamentals of nitroxide spin labeling through cutting-edge applications in chemistry, physics, materials science, molecular biology, and biomedicine. Nitroxides have earned their place as one of the most popular organic paramagnets due to their suitability as inhibitors of oxidative processes, as a means to polarize magnetic nuclei, and, in molecular biology, as probes and labels to understand molecular structures and dynamics AS DRAGS FOR CANCER AND OTHER DISEASES. Beginning with an overview of the basic methodology and nitroxides’ 145-year history, this book equips students with necessary background and techniques to undertake original research and industry work in this growing field.
Category: Science

Epr Spectroscopy

Author : Malte Drescher
ISBN : 9783642283468
Genre : Science
File Size : 76.79 MB
Format : PDF, ePub, Mobi
Download : 174
Read : 609

EPR Spectroscopy in Catalysis, by Sabine Van Doorslaer und Damien M. Murphy Radicals in Flavoproteins, by Erik Schleicher und Stefan Weber EPR Spectroscopy in Polymer Science, by Dariush Hinderberger EPR in Protein Science, by Intrinsically Disordered Proteins, by Malte Drescher Site-Directed Spin Labeling of Membrane Proteins, by Enrica Bordignon Structure and Dynamics of Nucleic Acids, by Ivan Krstić, Burkhard Endeward, Dominik Margraf, Andriy Marko und Thomas F Prisner New Directions in Electron Paramagnetic Resonance Spectroscopy on Molecular Nanomagnets, by J. van Slageren
Category: Science

Biomedical Epr Part A Free Radicals Metals Medicine And Physiology

Author : Sandra S. Eaton
ISBN : 0306485060
Genre : Medical
File Size : 78.37 MB
Format : PDF
Download : 366
Read : 610

Biomedical EPR – Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features: Free Radicals in Medicine Radicals in vivo and in Model Systems, and their Study by Spin Trapping In vivo EPR, including Oximetry and Imaging Time Domain EPR at Radio Frequencies EPR of Copper Complexes: Motion and Frequency Dependence Time Domain EPR and Electron Spin Echo Envelope Modulation About the Editors: Prof. Sandra S. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. Her research interests include distance measurements in proteins, EPR of metal ions in biological systems, electron spin relaxation times, and EPR instrumentation. The Eatons co-organize an annual EPR Symposium in Denver. Prof. Gareth R. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. His research interests include EPR instrumentation, distance measurements in proteins, EPR of metal ions in biological systems, and electron spin relaxation times. Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.
Category: Medical