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Molecular Biology Techniques

Author : Walt Ream
ISBN : 9780125839907
Genre : Medical
File Size : 43.82 MB
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter
Category: Medical

Molecular Microbiology Laboratory

Author : Walt Ream
ISBN : 9780123970442
Genre : Science
File Size : 49.94 MB
Format : PDF
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Molecular Microbiology Laboratory, second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles. Exposes students to the new molecular-based techniques Provides faculty with an authoritative, accessible resource for teaching protocols The only manual to incorporate writing exercises, presentation skills and tools for reading primary literature into the curriculum Based on a successful course for which the author won a teaching award New to this Edition: - Presents a real-world study of bacterial populations in the environment in the final experiment - Provides an overview of molecular biology in a new review chapter - Demonstrates how to design an experiment and how to interpret the results - Covers grant proposal writing and how panels review proposals - Presents guidance on public speaking and preparing PowerPoint presentations - Includes tutorials on three widely used software packages
Category: Science

Molecular Biology

Author : Burton E. Tropp
ISBN : 0763709166
Genre : Science
File Size : 32.87 MB
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The Third Edition of Molecular Biology: Genes to Proteins is a comprehensive guide through the basic molecular processes and genetic phenomena of both prokaryotic and eukaryotic cells. Designed for undergraduate and first year graduate students, this multi-purpose text has been completely updated to include many important advances made in the field of molecular biology. Maintaining the original structure-function approach evident in the first two editions of David Freifelder's classic text, this book provides students with an accessible overview of molecular biology before moving into more in-depth discussions. Broken into six sections, topics are strategically arranged to cover basic information that is necessary in understanding the more advanced topics focused on in later sections. Section 1 and 2, Protein Structure and Function and Nucleic Acids and Nucleoproteins provide the information required to Understand The chemical basis of molecular biology. Section 3, Genetics and Virology provides information required to Understand The biological basis of molecular biology. Section 4, DNA Metabolism explores DNA replication, repair, and recombination in bacteria, eukaryotes and archaeons. Section 5, RNA Synthesis and Processing examines mechanisms that bacteria, eukaryotes, and archaeons use to synthesize RNA, regulate RNA syntheses, and process transcripts to form mature RNA molecules. Section 6, Protein Synthesis describes the structure and function of the bacterial protein synthetic machinery in bacteria, eukaryotes and archaeons. View Preview Chapters in PDF of Molecular Biology: Genes to Proteins, Third Edition Today! Preview Chapter 1 Preview Chapter 2
Category: Science

Molecular Biology Techniques

Author : Sue Carson
ISBN : 9780123855442
Genre : Medical
File Size : 36.48 MB
Format : PDF
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Category: Medical

Using The Biological Literature

Author : Diane Schmidt
ISBN : 0824741714
Genre : Language Arts & Disciplines
File Size : 32.45 MB
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"Provides an in-depth review of current print and electronic tools for research in numerous disciplines of biology, including dictionaries and encyclopedias, method guides, handbooks, on-line directories, and periodicals. Directs readers to an associated Web page that maintains the URLs and annotations of all major Inernet resources discussed in this edition."
Category: Language Arts & Disciplines

Laboratory Investigations In Molecular Biology

Author : Steven A. Williams
ISBN : 0763733296
Genre : Science
File Size : 79.72 MB
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Laboratory Investigations in Molecular Biology presents well-tested protocols in molecular biology that are commonly used in currently active research labs. It is an ideal laboratory manual for college level courses in molecular biology. Because of the modular organization of the manual, laboratory courses can be assembled that would be ideal for science professionals, graduate students, undergraduate students and even advanced high school students in AP courses. The manual is also intended to be useful as a laboratory "bench reference". The experiments are designed to guide students through realistic research projects and to provide students with instruction in methods and approaches that can be immediately translated into research projects conducted in modern research laboratories. Although these experiments have been conducted and optimized over 20 years of teaching the New England Biolabs Molecular Biology Summer Workshops, they are real research projects, not "canned" experiments. Based on extensive teaching experience using these protocols, the authors have found that conducting these experiments as described in these protocols serves to effectively instruct students and science professions in the basic methods of molecular biology. An additional unique feature is that the protocols described in the manual are accompanied by available reagent kits that provide quality-tested, pre-packaged reagents to ensure the successful application of these protocols in a laboratory course setting.
Category: Science

Pcr Methods And Applications

Author :
ISBN : STANFORD:36105003101859
Genre : Polymerase chain reaction
File Size : 67.7 MB
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Category: Polymerase chain reaction

Biochemistry Laboratory

Author : Rodney F. Boyer
ISBN : 013604302X
Genre : Science
File Size : 71.62 MB
Format : PDF
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Your biochemistry lab course is an essential component in training for a career in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Biochemistry Laboratory: Modern Theory and Techniques covers the theories, techniques, and methodologies practiced in the biochemistry teaching and research lab. Instead of specific experiments, it focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.
Category: Science