High Performance Liquid Chromatography

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High Performance Liquid Chromatography

Author : Csaba Horváth
ISBN : 9781483261843
Genre : Science
File Size : 30.38 MB
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High-Performance Liquid Chromatography: Advances and Perspectives, Volume 5 presents the applications of high-performance liquid chromatography to the analysis and purification of biopolymers. The book, composed of three chapters, provides a detailed description of silica gel-supported stationary phases; tackles biospecific interaction chromatography, a tool in the study of complex carbohydrates and as an industrial separation process in biotechnology; and discusses the potential advantages of displacement chromatography for multicomponent separations. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good source of information.
Category: Science

High Performance Liquid Chromatography

Author : Sandie Lindsay
ISBN : 0471931152
Genre : Science
File Size : 24.58 MB
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Part of a series of textbooks concerned with the training, continuing education and updating of all technical staff concerned with analytical chemistry, this volume explores a powerful chromatographic technique which has arisen from research carried out on liquid and gas chromatography.
Category: Science

High Performance Liquid Chromatography

Author : W.J. Lough
ISBN : 0751400769
Genre : Science
File Size : 57.39 MB
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High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.
Category: Science

High Performance Liquid Chromatography

Author : Mary T. Gilbert
ISBN : 9781483281292
Genre : Science
File Size : 52.61 MB
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High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.
Category: Science

High Performance Liquid Chromatography In Phytochemical Analysis

Author : Monika Waksmundzka-Hajnos
ISBN : 1420092618
Genre : Health & Fitness
File Size : 33.15 MB
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The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp
Category: Health & Fitness

High Performance Liquid Chromatography Hplc

Author : Yuegang Zuo
ISBN : 1629488542
Genre : Technology & Engineering
File Size : 90.50 MB
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High-performance liquid chromatography (HPLC) has emerged as the most powerful and versatile separation and analytical method. This book covers not only the conventional HPLC techniques but also the new developments, novel separation modes, column technology, as well as procedures and practices, particularly the advanced applications of HPLC in the fields of pharmaceutical, clinical, bioanalytical and food sciences.
Category: Technology & Engineering

High Performance Liquid Chromatography Evolution Of Liquid Chromatography

Author : Csaba Horváth
ISBN : MINN:31951000465621S
Genre : High performance liquid chromatography
File Size : 45.45 MB
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High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis. The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient e ...
Category: High performance liquid chromatography

Practice Of High Performance Liquid Chromatography

Author : Heinz Engelhardt
ISBN : 9783642692253
Genre : Science
File Size : 50.36 MB
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During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).
Category: Science

High Performance Liquid Chromatography

Author : Brown PR.
ISBN : UOM:39015038990811
Genre : Science
File Size : 60.57 MB
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Detectors for Liquid Chromatography Edited by Edward S. Yeung Written by an expert in the field, this comprehensive guide explains the basic principles behind detector response instrumentation and selected applications. Early chapters cover absorption detectors for high performance liquid chromatography, FTIR detection, indirect absorbance detectors, fluorometric detection, and polarimetric detectors. Coverage continues with detection based on electrical and electrochemical measurements, mass spectrometry as an online detector for HPLC, and miscellaneous methods. 1986 0 471-82169-1 366 pp. Small Bore High Performance Liquid Chromatography Edited by Raymond R. W. Scott A state-of-the-art guide that demonstrates how to design, construct, and pack optimized small bore columns, the center of any chromatography system. Case examples show the use of these columns for high resolution, very fast analysis, and special methods for molecular weight determinations. Applications from a wide range of industrial and forensic analyses aid in developing sophistication in a number of useful techniques. The book provides essential information on topics such as calculating the minimum column radius, detectors, and molecular diffusion. Includes 92 illustrations and 14 tables to enhance explanations of microbore HPLC methods. 1984 0 471-80052-X 271 pp. Reversed-Phase High Performance Liquid Chromatography: Theory, Practice, and Biomedical Applications Ante M. Krstulovic and Phyllis R. Brown Reversed-phase liquid chromatography has increased tremendously in popularity over the past ten years. Estimates show that more than 80% of all HPLC separations are performed using this technique. This book covers both theoretical aspects of RPLC and practical information needed in diverse areas of research; it also contains a review of the RPLC applications in the biomedical/biochemical field, with references and collateral readings. Material is presented in a practical, problem-solving manner and should be immensely useful in theoretical aspects of RPLC and all areas of scientific research, particularly the biomedical/biochemical field, where RPLC has made its largest impact. 1982 0 471-05369-4 296 pp.
Category: Science

High Performance Liquid Chromatography Capillary Electrophoresis

Author : Andrea Weston
ISBN : 0080534163
Genre : Science
File Size : 28.75 MB
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HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques Applicable to a wide variety of separation problems Covers basic concepts governing any separation as well as instrumentation and how to use it Helps the user to obtain optimal resolution in minimal time Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation Includes information on upcoming trends such as miniaturization Major concepts in each chapter are organized to allow access to information easily and quickly Contains practical bibliography for accessing the literature
Category: Science

The Hplc Expert

Author : Stavros Kromidas
ISBN : 9783527336814
Genre : Science
File Size : 81.21 MB
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The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.
Category: Science

High Performance Liquid Chromatography In Pesticide Residue Analysis

Author : Tomasz Tuzimski
ISBN : 9781466568822
Genre : Science
File Size : 54.44 MB
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HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure, the chromatographer should possess both the practical and theoretical skills required to perform HPLC experiments correctly and to obtain reliable, repeatable, and r
Category: Science

High Performance Liquid Chromatography And Lipids

Author : William W. Christie
ISBN : UOM:39015053342641
Genre : Science
File Size : 22.65 MB
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A comprehensive guide to lipids and lipid qnalysis using high-performance liquid chromatography. The author covers the construction and occurrence of various lipids and their functions. The book introduces the values of high-performance liquid chromatography, its theoretical considerations and the use of equipment, and discusses the separation of both simple and complex lipid classes. In combining the theoretical and practical sides of lipid anlaysis this book will be of immense value to all those involved in lipid research.
Category: Science

Forensic Applications Of High Performance Liquid Chromatography

Author : Shirley Bayne
ISBN : 9781439882597
Genre : Law
File Size : 37.78 MB
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Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic App
Category: Law

High Performance Liquid Chromatography And Mass Spectrometry Of Porphyrins Chlorophylls And Bilins

Author : Chang Kee Lim
ISBN : 9789814497671
Genre : Science
File Size : 65.15 MB
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The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field. Contents:Structure, Distribution, Biosynthesis and FunctionHigh-Performance Liquid Chromatography of PorphyrinsMass Spectrometry of PorphyrinsPorphyrin Profiles in Blood, Urine and Faeces by HPLC and HPLC/ESI-MSIsolation and Characterization of Protoporphyrin Glycoconjugates from Harderian Glands of Rodents by HPLC and HPLC/ESI-MSHPLC and HPLC/MS of Chlorophyll and Related CompoundsHPLC and HPLC/MS of Bilins of Animal and Plant OriginFuture Directions of HPLC and Mass spectrometry of Tetrapyrroles Readership: Analytical biochemists, clinical biochemists, researchers in tetrapyrolle chemistry and biochemistry, plant scientists, pharmaceutical chemists. keywords:Porphyrins;Chlorophylls;Bile Pigments;Bilins;High-Performance Liquid Chromatography of Porphyrins;Mass Spectrometry of Porphyrins;Tandem MS/MS of Tetrapyrroles
Category: Science