Embryonic Stem Cell Protocols

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Human Embryonic Stem Cell Protocols

Author : Kursad Turksen
ISBN : 9781597450461
Genre : Computers
File Size : 23.28 MB
Format : PDF, ePub
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Human Embryonic Stem Cell Protocols contributes to progress in the field by investigating human embryonic stem cells (hES) and increasing our understanding of the regulation of their self-renewal capacity, commitment and differentiation along various lineages, and their potential utility in regenerative medicine applications. These hard-won protocols, all generously shared by experts in this field, support the continued isolation of additional hES lines as well as the development of improved culture conditions, countering the view that hES cells are difficult to maintain and use.
Category: Computers

Nonhuman Embryonic Stem Cell Protocols

Author : Kursad Turksen
ISBN : 9781597450362
Genre : CD-ROMs
File Size : 34.56 MB
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It is clear that the potentials of assessing embryonic stem (ES) cells in regenerative medicine applications is evident in the ever-increasing publications in which ES cell biology and differentiation along diverse lineages appear in the academic as well as the popular press. These two new volumes present important advances in the field since the publication of Embryonic Stem Cells: Methods and Protocols four years ago. These two volumes provide an update and complement to that volume, focusing on ES cells recently isolated from other/non-mouse species. Each volume contains numerous updates, more advanced approaches, and completely new protocols for the use of ES cells in studies of diverse cell lineages. These two volumes will surely expand the experimental repetoires of both experts and novices in the field.
Category: CD-ROMs

Embryonic Stem Cell Protocols

Author : Kursad Turksen
ISBN : 9781597450379
Genre : CD-ROMs
File Size : 69.37 MB
Format : PDF, ePub, Mobi
Download : 458
Read : 767

It is clear that the potentials of assessing embryonic stem (ES) cells in regenerative medicine applications is evident in the ever-increasing publications in which ES cell biology and differentiation along diverse lineages appear in the academic as well as the popular press. These two new volumes present important advances in the field since the publication of Embryonic Stem Cells: Methods and Protocols four years ago. These two volumes provide an update and complement to that volume, focusing on ES cells recently isolated from other/non-mouse species. Each volume contains numerous updates, more advanced approaches; and completely new protocols for the use of ES cells in studies of diverse cell lineages. These two volumes will surely expand the experimental repertoires of both experts and novices in the field.
Category: CD-ROMs

Embryonic Stem Cells

Author : Michael S. Kallos
ISBN : 9789533076324
Genre : Science
File Size : 28.53 MB
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The ultimate clinical implementation of embryonic stem cells will require methods and protocols to turn these unspecialized cells into the fully functioning cell types found in a wide variety of tissues and organs. In order to achieve this, it is necessary to clearly understand the signals and cues that direct embryonic stem cell differentiation. This book provides a snapshot of current research on the differentiation of embryonic stem cells to a wide variety of cell types, including neural, cardiac, endothelial, osteogenic, and hepatic cells. In addition, induced pluripotent stem cells and other pluripotent stem cell sources are described. The book will serve as a valuable resource for engineers, scientists, and clinicians as well as students in a wide range of disciplines.
Category: Science

Human Embryonic Stem Cells

Author : Stephen Sullivan
ISBN : 0470511591
Genre : Science
File Size : 28.75 MB
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With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The Practical Handbook contains the first centralised collection of methods used in human embryonic stem cell biology. The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. This allows an even greater number of opportunities for those interested in purusing work in pluripotent stem cells, disease modelling, and other aspects of basic regenerative medicine research. The novel and useful focus of this book sets it apart from other available books: Compares and evaluates the protocols used in leading laboratories working on human embryonic stem cells Centred solely on practical protocols for human (not mouse) embryonic stem cell research Includes extensive troubleshooting sections Addresses the different proclivities and behaviours of individual human embryonic cell lines Contains techniques currently known only to a small number of specialised laboratories worldwide This handbook represents an essential source of up-to-date practical information for all cell and developmental biologists working with human embryonic stem cells or wishing to enter the field. It is also essential reading for clinical researchers in areas such as diabetes, cardiovascular disease, and neurological diseases. Praise from the reviews: "...a highly readable and useful book... A notable feature of the book is its air of openness and honesty... This book... will help many to navigate the uncharted waters of human embryonic stem cell biology." BRITISH SOCIETY FOR CELL BIOLOGY "... the imaginative solutions in this book can inspire us to get past our most frustrating limitations." CELL STEM CELL "... the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human pluripotent stem cells..." REGENERATIVE MEDICINE "In this fast-moving field, this [handbook] will help drive advances of more and more researchers." DIFFERENTIATION "...a valuable resource for seasoned and novice researchers... an excellent addition to the reference collection of any medical library or research laboratory." THE AMERICAN MEDICAL ASSOCIATION
Category: Science

Stem Cell Tools And Other Experimental Protocols

Author :
ISBN : 9780080469645
Genre : Technology & Engineering
File Size : 38.77 MB
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This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering Presents the latest most innovative technologies Addresses therapeutic relevance including FDA compliance and tissue engineering
Category: Technology & Engineering

Protocols For Adult Stem Cells

Author : Irina M. Conboy
ISBN : 1493961047
Genre : Science
File Size : 28.22 MB
Format : PDF
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Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body’s natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so far are generated and regenerated from stem cells, it has been an important frst step to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Much of this work, and that in the classical model org- isms, has helped defne the aspects of the stem cell environment or niche that are crucial for both growth and differentiation, and these studies have moved in vivo at increasingly higher resolution. Importantly, the in vivo niche is a current target for bioengineering the matrix and signaling factors. Herein, we present methods for studying six types of mammalian stem cells, m- mary, neural, mesenchymal, endothelial, dendritic, and muscle.
Category: Science

Gene Targeting And Embryonic Stem Cells

Author : Jim Mcwhir
ISBN : 9781135319427
Genre : Science
File Size : 51.11 MB
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Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.
Category: Science

Human Embryonic Stem Cell Protocols

Author : Kursad Turksen
ISBN : 9781588294975
Genre : Science
File Size : 78.74 MB
Format : PDF, ePub, Docs
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A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Category: Science

Hematopoietic Stem Cell Protocols

Author : Kevin D. Bunting
ISBN : 9781588298683
Genre : Science
File Size : 65.42 MB
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This revised edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. The book provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations, and offers a variety of transplantation approaches to measure HSC function in vivo. This is a much needed technical resource in the critically important field of stem cell investigation.
Category: Science

Stem Cell Assays

Author : Mohan C. Vemuri
ISBN : 9781588297440
Genre : Medical
File Size : 82.88 MB
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The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.
Category: Medical

Neural Stem Cells

Author : Leslie P. Weiner
ISBN : 9781588298461
Genre : Social Science
File Size : 54.49 MB
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Many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. This second edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. This is an excellent source of information and inspiration.
Category: Social Science

Human Embryonic Stem Cell Protocols

Author : ANONIMO
ISBN : 1617376000
Genre : Medical
File Size : 38.49 MB
Format : PDF, Docs
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A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biologya series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Category: Medical

Culture Of Human Stem Cells

Author : R. Ian Freshney
ISBN : 0470167513
Genre : Science
File Size : 90.38 MB
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This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.
Category: Science

Local Cells Global Science

Author : Aditya Bharadwaj
ISBN : 9780415396097
Genre : Medical
File Size : 33.18 MB
Format : PDF
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One of the first studies of an exciting new development in global biotechnology, this cutting edge text examines the extent of the transnational movements of tissues, stem cells, and expertise, in the developing governance framework of India. Documenting the impact of local and global governance frames on the everyday conduct of research, this groundbreaking book traces the journey of ‘spare’ human embryos in IVF clinics to public and private laboratories engaged in isolating stem cells for potential therapeutic application. The discussion also examines the gender dimension as a potential site for exploitation in the sourcing of embryonic and other biogenic materials, and suggests that a moral economy has developed in which the ethical values of the global 'North' support and encourage the donation of abundant and ethically ‘neutral’ embryos by the 'South'. This unique exploration is grounded in an empirical, multi-sited ethnographic study that takes a thoroughly comparative analysis of the ethical, religious and social issues in Europe, the United States, and organ donations already prevalent in India. In this theoretically-sensitive analysis, the authors use the resources of social anthropology and the social sciences in an innovative text which will appeal to postgraduates and professionals in the areas of STS studies, genetics, bioethics, and anthropology.
Category: Medical

In Vitro Differentiation Of T Cells

Author : Shin Kaneko
ISBN : 1493997300
Genre : Science
File Size : 78.53 MB
Format : PDF
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This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.
Category: Science

Handbook Of Stem Cells

Author : Anthony Atala
ISBN : 9780123859433
Genre : Science
File Size : 50.85 MB
Format : PDF
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New discoveries in the field of stem cells increasingly dominate the news and scientific literature revealing an avalanche of new knowledge and research tools that are producing therapies for cancer, heart disease, diabetes, and a wide variety of other diseases that afflict humanity. The Handbook of Stem Cells integrates this exciting area of life science, combining in two volumes the requisites for a general understanding of adult and embryonic stem cells. Organized in two volumes entitled Pluripotent Stem Cells and Cell Biology and Adult and Fetal Stem Cells, this work contains contributions from the world’s experts in stem cell research to provide a description of the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations as well as a the latest information of what is known about each specific organ system. Provides comprehensive coverage on this highly topical subject Contains contributions by the foremost authorities and premiere names in the field of stem cell research Companion website - http://booksite.elsevier.com/9780123859426/ - contains over 250 color figures in presentation format
Category: Science

Differentiating Mouse Embryonic Stem Cells To Use For Implantation As A Therapy For Spinal Cord Injury

Author : Darren B Sarte
ISBN : OCLC:1182025897
Genre :
File Size : 73.60 MB
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In the United States, an estimated 6 million individuals live with paralysis, 23% of these incidents being due to spinal cord injury. Following an injury to the central nervous system there are events that occur to segregate inflammation from the surrounding healthy tissue, this is characterized by the formation of the astrocyte scar. Once the astrocyte scar has formed, regrowth of axon across the injured area is greatly impeded if not impossible. Using mouse embryonic stem cells we aim to generate a population of neural progenitor following a differentiation protocol using retinoic acid and sonic hedgehog. Following differentiation in vitro we fixed cells to cover slips and stained using immunohistochemistry to identify if we cell markers designating the cell lineage. Our data show that embryonic stem cells that were exposed to the differentiation protocol were positive for the neural progenitor marker Nestin. We also wanted to determine that we could influence the development of these progenitors toward either astrocyte or neuron lineages using small molecules. Following the differentiation protocol we further treated cells with FGF or SUN11602, an FGF mimetic, to generate astrocytes or DAPT, a Notch inhibitor, to generate neurons. From our initial results, FGF ix was capable of generating a large population of astrocytes although not homogeneous. When using the SUN11602 depending on the concentration of retinoic acid used in the differentiation protocol we either saw no differentiation for the low concentration of retinoic acid or we saw the generation of mostly neurons for the higher concentration of retinoic acid used. A similar result was observed for cells that were treated with DAPT, when differentiating with lower concentrations of retinoic acid we saw very little generation of astrocytes or neurons and when higher concentrations were used we saw some generation of neurons. We will continue in designing a more robust differentiation protocol, which will eventually lead us to our next goal of implanting these cells in vivo using a mouse model for spinal cord injury.
Category:

Embryonic Stem Cells

Author :
ISBN : 0080471129
Genre : Medical
File Size : 88.15 MB
Format : PDF, Mobi
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This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. * Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering * Presents the latest most innovative technologies * Addresses therapeutic relevance including FDA compliance and tissue engineering
Category: Medical