THE IMMUNOASSAY HANDBOOK FOURTH EDITION THEORY AND APPLICATIONS OF LIGAND BINDING ELISA AND RELATED TECHNIQUES

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The Immunoassay Handbook

Author : David Wild
ISBN : 9780080970387
Genre : Science
File Size : 22.48 MB
Format : PDF, Docs
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The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing. www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. Provides a unique mix of theory, practical advice and applications, with numerous examples Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Category: Science

Handbook Of Immunoassay Technologies

Author : Sandeep K. Vashist
ISBN : 9780128117941
Genre : Medical
File Size : 45.50 MB
Format : PDF, ePub, Docs
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Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text. Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules. Provides a valuable increase of understanding of cellular and biomedical functions Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings Discusses all multifarious aspects of immunoassays Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays
Category: Medical

Bioconjugate Techniques

Author : Greg T. Hermanson
ISBN : 9780123822406
Genre : Science
File Size : 53.9 MB
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Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented
Category: Science

Practical Immunology

Author : Frank C. Hay
ISBN : 9781405146739
Genre : Medical
File Size : 41.71 MB
Format : PDF, Docs
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Practical Immunology is a basic text aimed at immunology students and researchers at all levels who need a comprehensive overview of the methodology of immunology. The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice and it has always been the aim of this book to educate researchers in the design and performance of complex techniques. It will appeal to students of immunlogy, graduate students embarking on bench science, or specialised immunologists who need to use an immunological technique outside their sphere of expertise. The definitive lab "bench book". A one stop resource. Techniques explained from first principles. Basic forms of apparatus described in detail. Totally revised with new user friendly layout to aid use in the lab. Includes useful hints and tips.
Category: Medical

Lateral Flow Immunoassay

Author : Raphael Wong
ISBN : 1597452408
Genre : Science
File Size : 80.79 MB
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Due to the simplicity, relative accuracy, fast result reporting, and user-friendliness of lateral flow immunoassay, its use has undergone tremendous growth in the diagnostic industry in the last few years. Such technology has been utilized widely and includes pregnancy and woman's health determination, cardiac and emergency conditions monitoring and testing, infectious disease including Flu screening, cancer marker screening, and drugs abuse testing. This book covers the scope of utilization, the principle of the technology, the patent concerns, information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the general scientific community.
Category: Science

Medical Immunology

Author : Tristram G. Parslow
ISBN : UOM:39015053123843
Genre : Medical
File Size : 41.96 MB
Format : PDF
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* Emphasizes both the basic and clinical aspects of immunology that promotes understanding of core concepts and provides clinical correlations to medical practice * Logical progression from normal immune function to laboratory abnormalities and clinical diseases* Problem-oriented approach to clinical disorders caused by immunological disruption
Category: Medical

Handbook Of Human Immunology Second Edition

Author : Maurice R.G. O'Gorman
ISBN : 1420003712
Genre : Medical
File Size : 56.81 MB
Format : PDF, ePub
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Since the publication of the first edition of the Handbook of Human Immunology in 1997, major scientific achievements have directly contributed to an increased understanding of the complexities of the human immune system in health and disease. Whether as a result of the sequencing of the entire human genome, or of technological advancements, several new components of the immune system have been revealed, along with new technologies for their measurement and evaluation. Major breakthroughs in the field include an increase in the number of recognized "clusters of differentiation" on the surface of leukocytes and associated cells, the establishment of a chemokine and chemokine receptor nomenclature system, the discovery of more than 30 lymphokines, and humanized monoclonal antibody therapy as a staple of pharmacologic armamentarium Modeling the previous edition, the text begins with an overview of the immune system, focusing on the role of cell receptors, accessory molecules, and cytokines in immune responses and immunological disorders. It then presents a practical, easy-to-read chapter on "statistics in immunological testing"—an invaluable asset for interpreting test results, validating new tests, and developing reference ranges. Simultaneously, the text emphasizes clinically relevant immunological parameters and clarifies the basic principles underlying immune system assays, and applications and interpretations of immune tests. A complete guide to molecular and cellular immunology for practicing clinicians, clinical laboratory professionals, and students, this resource combines basic explanations of laboratory tests with more than 100 tables full of references, and up-to-date information on new developments in immunogenetics.
Category: Medical

Rna Methodologies

Author : Robert E. Farrell, Jr.
ISBN : 9780080454764
Genre : Science
File Size : 74.65 MB
Format : PDF, Kindle
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This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Category: Science

Molecular Biomethods Handbook

Author : John M. Walker
ISBN : 1603273751
Genre : Science
File Size : 43.84 MB
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Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Category: Science

A Practical Guide To Elisa

Author : D. M. Kemeny
ISBN : UOM:39015021858249
Genre : Science
File Size : 24.76 MB
Format : PDF
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This practical and readable handbook is written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay. Basic aspects of immunoassays and assay design are discussed. The explanation of ELISA, describing the reagents used, how to calibrate the equipment and how to interpret results, encourages understanding of this technique. The chapter on "Trouble Shooting" enables the user to identify and rectify problems, thus increasing his knowledge and ability so that the recipe section at the end of the book may be used to full advantage.
Category: Science