MOLECULAR BIOLOGY TECHNIQUES AN INTENSIVE LABORATORY COURSE

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Molecular Biology Techniques

Author : Walt Ream
ISBN : 9780080536828
Genre : Science
File Size : 62.39 MB
Format : PDF
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter
Category: Science

Molecular Microbiology Laboratory

Author : Walt Ream
ISBN : 9780123970442
Genre : Science
File Size : 69.84 MB
Format : PDF
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Molecular Microbiology Laboratory, second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles. Exposes students to the new molecular-based techniques Provides faculty with an authoritative, accessible resource for teaching protocols The only manual to incorporate writing exercises, presentation skills and tools for reading primary literature into the curriculum Based on a successful course for which the author won a teaching award New to this Edition: - Presents a real-world study of bacterial populations in the environment in the final experiment - Provides an overview of molecular biology in a new review chapter - Demonstrates how to design an experiment and how to interpret the results - Covers grant proposal writing and how panels review proposals - Presents guidance on public speaking and preparing PowerPoint presentations - Includes tutorials on three widely used software packages
Category: Science

Molecular Biology Techniques

Author : Heather Miller
ISBN : 9780123855459
Genre : Science
File Size : 25.51 MB
Format : PDF, Kindle
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Category: Science

Basic Techniques In Molecular Biology

Author : Stefan Surzycki
ISBN : 9783642569685
Genre : Science
File Size : 59.76 MB
Format : PDF, Mobi
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This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Category: Science

Human Molecular Biology Laboratory Manual

Author : Stefan Surzycki
ISBN : 9780470999240
Genre : Science
File Size : 58.42 MB
Format : PDF, Docs
Download : 236
Read : 526

Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual’s distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Category: Science

Manipulation And Expression Of Recombinant Dna

Author : Sue Carson
ISBN : 9780080456546
Genre : Science
File Size : 21.44 MB
Format : PDF, ePub, Mobi
Download : 600
Read : 1312

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Category: Science

Wilson And Walker S Principles And Techniques Of Biochemistry And Molecular Biology

Author : Andreas Hofmann
ISBN : 9781108365253
Genre : Science
File Size : 60.23 MB
Format : PDF, Docs
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Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.
Category: Science

Molecular Biology

Author : Nancy Craig
ISBN : 9780199658572
Genre : Science
File Size : 61.38 MB
Format : PDF, ePub
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This text offers a fresh, distinctive approach to the teaching of molecular biology that reflects the challenge of teaching a subject that is in many ways unrecognizable from the molecular biology of the 20th century - a discipline in which our understanding has advanced immeasurably, but about which many questions remain to be answered. With a focus on key principles, this text emphasizes the commonalities that exist between the three kingdoms of life, giving students an accurate depiction of our current understanding of the nature of molecular biology and the differences that underpin biological diversity.
Category: Science

Molecular Biology

Author : Burton E. Tropp
ISBN : 9780763786632
Genre : Science
File Size : 33.17 MB
Format : PDF, ePub, Docs
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"Molecular Biology: Genes to Proteins is a guide through the basic molecular processes and genetic phenomena of both prokaryotic and eukaryotic cells. Written for the undergraduate and first year graduate students within molecular biology or molecular genetics, the text has been updated with the latest data in the field. It incorporates a biochemical approach as well as a discovery approach that provides historical and experimental information within the context of the narrative."--Publisher.
Category: Science