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Introduction To Modern Liquid Chromatography

Author : Lloyd R. Snyder
ISBN : 9781118210390
Genre : Science
File Size : 54.20 MB
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The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today theleading technique for chemical analysis and related applications,with an ability to separate, analyze, and/or purify virtually anysample. Snyder and Kirkland's Introduction to Modern LiquidChromatography has long represented the premier reference toHPLC. This Third Edition, with John Dolan as added coauthor,addresses important improvements in columns and equipment, as wellas major advances in our understanding of HPLC separation, ourability to solve problems that were troublesome in the past, andthe application of HPLC for new kinds of samples. This carefully considered Third Edition maintains thestrengths of the previous edition while significantly modifying itsorganization in light of recent research and experience. The textbegins by introducing the reader to HPLC, its use in relation toother modern separation techniques, and its history, then leadsinto such specific topics as: The basis of HPLC separation and the general effects ofdifferent experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography,gradient elution, two-dimensional separation, and othertechniques Computer simulation, qualitative and quantitative analysis, andmethod validation and quality control The separation of large molecules, including both biologicaland synthetic polymers Chiral separations, preparative separations, and samplepreparation Systematic development of HPLC separations—new to thisedition Troubleshooting tricks, techniques, and case studies for bothequipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, fromnovices to experts, Introduction to Modern LiquidChromatography, Third Edition offers the most up-to-date,comprehensive, and accessible survey of HPLC methods andapplications available.
Category: Science

Practical Hplc Method Development

Author : Lloyd R. Snyder
ISBN : 9781118591512
Genre : Science
File Size : 51.38 MB
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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.
Category: Science

Analysis And Analyzers

Author : Béla G. Lipták
ISBN : 9781315353241
Genre : Science
File Size : 85.78 MB
Format : PDF
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The Instrument and Automation Engineers’ Handbook (IAEH) is the #1 process automation handbook in the world. Volume two of the Fifth Edition, Analysis and Analyzers, describes the measurement of such analytical properties as composition. Analysis and Analyzers is an invaluable resource that describes the availability, features, capabilities, and selection of analyzers used for determining the quality and compositions of liquid, gas, and solid products in many processing industries. It is the first time that a separate volume is devoted to analyzers in the IAEH. This is because, by converting the handbook into an international one, the coverage of analyzers has almost doubled since the last edition. Analysis and Analyzers: Discusses the advantages and disadvantages of various process analyzer designs Offers application- and method-specific guidance for choosing the best analyzer Provides tables of analyzer capabilities and other practical information at a glance Contains detailed descriptions of domestic and overseas products, their features, capabilities, and suppliers, including suppliers’ web addresses Complete with 82 alphabetized chapters and a thorough index for quick access to specific information, Analysis and Analyzers is a must-have reference for instrument and automation engineers working in the chemical, oil/gas, pharmaceutical, pollution, energy, plastics, paper, wastewater, food, etc. industries. About the eBook The most important new feature of the IAEH, Fifth Edition is its availability as an eBook. The eBook provides the same content as the print edition, with the addition of thousands of web addresses so that readers can reach suppliers or reference books and articles on the hundreds of topics covered in the handbook. This feature includes a complete bidders' list that allows readers to issue their specifications for competitive bids from any or all potential product suppliers.
Category: Science

High Performance Gradient Elution

Author : Lloyd R. Snyder
ISBN : 9780470055519
Genre : Science
File Size : 44.76 MB
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Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.
Category: Science

Chromatographic Methods

Author : A. Braithwaite
ISBN : 9789400940932
Genre : Juvenile Nonfiction
File Size : 63.7 MB
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In recent years the techniques of chromatography have progressed rapidly. However, the aims and objectives of the First Edition, as quoted below, are just as relevant today as they undoubtedly were in 1963. 'The various methods of separating mixtures which are grouped under the general name chromatography are now well known and widely used. Since the inception of chromatography as a column technique in 1903, the principal landmarks in its progress have been its virtual rediscovery in the 1930s, the invention of synthetic resins in 1935, the introduction of paper chromatography in the early 1940s and finally, the development of gas solid and gas liquid chromatography in the late 1940s and early 1950s. Subsequent expansion in the use of chromatographic methods has been rapid and continuous, with the result that in the last 15 years a substantial volume of literature on the subject has appeared, dealing not only with particular separations but also in much specific detail with improvements in technique.
Category: Juvenile Nonfiction

Essentials In Modern Hplc Separations

Author : Serban C. Moldoveanu
ISBN : 9780123850133
Genre : Science
File Size : 41.87 MB
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This book discusses in a systematic manner the role of separation in HPLC, the types and characteristics of stationary phases and of mobile phases used in this technique, as well as other factors influencing the separation. The selection process of stationary and mobile phase for a specific separation is described as related to the physico-chemical characteristics of the molecules to be separated and of their matrix. All these subjects are discussed from the point of view of the new developments in HPLC. The book also includes a part presenting the practice of modern HPLC as necessary for applications, particularly related to the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, etc. Gives a clear presentation of notions and conceptsDiscusses key parameters in HPLC separationIncludes modern developments in HPLCDescribes interrelation between various HPLC features (solvent pressure, separation, detection)Includes a large number of references.
Category: Science

Chromatography And Separation Science

Author : Satinder Ahuja
ISBN : 9780080501123
Genre : Science
File Size : 23.10 MB
Format : PDF
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The basic objectives of this book are to: provide basic information on chromatography and separation science; show how simple extraction and partition processes provide the basis for development of chromatography and separation science; describe the role of chromatography and separation science in various fields; discuss the role of chromatography and separation science in development of new methodology; and present new evolving methods and how to select an optimum method. · The book covers the fundamental physical and chemical phenomena involved in separations · Provides a concise overview of the basics of transport phenomena and thermodynamics · Shows the importance of chromatography within separation science
Category: Science