GLYCOSYLATION 3 CELL ENGINEERING

Download Glycosylation 3 Cell Engineering ebook PDF or Read Online books in PDF, EPUB, and Mobi Format. Click Download or Read Online button to GLYCOSYLATION 3 CELL ENGINEERING book pdf for free now.

Glycosylation

Author : Mohamed Al-Rubeai
ISBN : 1402007337
Genre : Science
File Size : 23.37 MB
Format : PDF, Mobi
Download : 475
Read : 238

The analysis and modification of glycans of recombinant proteins continues to be a challenging area of research. This text provides reviews of advances in the subject and an assessment of state-of-the-art methodology and the various approaches for the improvement of glycoprotein production.
Category: Science

Cell Culture Engineering

Author : Wei-Shu Hu
ISBN : 9783540340072
Genre : Science
File Size : 52.21 MB
Format : PDF, ePub
Download : 314
Read : 293

Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.
Category: Science

Glycotechnology

Author : E. G. Berger
ISBN : 9781461552574
Genre : Science
File Size : 52.27 MB
Format : PDF, Kindle
Download : 946
Read : 593

Glycotechnology brings together in one place important contributions and up-to-date research results in this fast moving area. Glycotechnology serves as an excellent reference, providing insight into some of the most challenging research issues in the field.
Category: Science

Antibody Expression And Production

Author : Mohamed Al-Rubeai
ISBN : 940071257X
Genre : Medical
File Size : 82.19 MB
Format : PDF
Download : 119
Read : 264

Engineered antibodies currently represent over 30% of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in microbial and animal cell cultures and in transgenic animals and plants. There is a strong focus on those issues related to the production of intrabodies, bispecific antibody and antibody fragments and also to novel applications in cancer immunotherapy.
Category: Medical

The Metabolic Pathway Engineering Handbook

Author : Christina Smolke
ISBN : 1439802971
Genre : Medical
File Size : 40.93 MB
Format : PDF, ePub, Mobi
Download : 459
Read : 710

This first volume of the Metabolic Pathway Engineering Handbook provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus Subtilis, Streptomyces, filamentous fungi, and mammalian cells using cell culture. Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set. For information on the complete handbook visit Cat No. 3923
Category: Medical

Animal Cell Culture

Author : Mohamed Al-Rubeai
ISBN : 9783319103204
Genre : Medical
File Size : 55.30 MB
Format : PDF, ePub, Docs
Download : 119
Read : 1047

​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.
Category: Medical

Glycosylation

Author : Mohamed Al-Rubeai
ISBN : 9781402007330
Genre : Science
File Size : 76.2 MB
Format : PDF, ePub, Docs
Download : 148
Read : 1081

The analysis and modification of glycans of recombinant proteins continues to be a challenging area of research. This text provides reviews of advances in the subject and an assessment of state-of-the-art methodology and the various approaches for the improvement of glycoprotein production.
Category: Science

Animal Cell Biotechnology

Author : Hansjörg Hauser
ISBN : 9783110278965
Genre : Science
File Size : 49.10 MB
Format : PDF, Docs
Download : 926
Read : 1189

This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.
Category: Science

Cell Engineering

Author : Mohamed Al-Rubeai
ISBN : 0792357906
Genre : Animal cell biotechnology
File Size : 44.45 MB
Format : PDF, ePub
Download : 460
Read : 466

Category: Animal cell biotechnology