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Author : Jeffery M. Becker
ISBN : 9780323159135
Genre : Science
File Size : 23.64 MB
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Biotechnology: A Laboratory Course is a series of laboratory exercises demonstrating the in-depth experience and understanding of selected methods, techniques, and instrumentation used in biotechnology. This manual is an outgrowth of an introductory laboratory course for senior undergraduate and first year graduate students in the biological sciences at The University of Tennessee. This book is composed of 19 chapters and begins with some introductory notes on record keeping and safety rules. The first exercises include pH measurement, the use of micropipettors and spectrophotometers, the concept of aseptic technique, and preparation of culture media. The subsequent exercises involve the application of the growth curve, the isolation, purification, and concentration of plasmid DNA from Escherichia coli, and the process of agarose gel electrophoresis. Other exercises include the preparation, purification, and hybridization of probe, the transformation of Saccharomyces cerevisiae, the transformation of E. coli by plasmid DNA, and the principles and applications of protein assays. The final exercises explore the ?-galactosidase assay and the purification and determination of ?-galactosidase in permeabilized yeast cells. This book is of great value to undergraduate biotechnology and molecular biology students.
Category: Science


Author : J. Kirk Brown
ISBN : 0983239630
Genre :
File Size : 39.56 MB
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Ana Techniqs In Biotechnology

Author : Goutam Bhowmik
ISBN : 0070700133
Genre : Biotechnology
File Size : 30.86 MB
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'Analytical Techniques in Biotechnology' by Bhowmik and Bose offers a unique compendium of fundamental experiments, which forms the crucial foundation to understand this contemporary subject that has enormous impact on many other branches of life sciences. In addition to its simple and lucid language, the main focus of the book is to equip a beginner with the skill and ability required to conduct independent experimentation and research in laboratories. Carefully structured to cover the complete spectra of subjects under the umbrella of 'Biotechnology', this book is sure to prove a ready reference for students, faculties and researchers, all alike!
Category: Biotechnology

Biotechnology Proteins To Pcr

Author : David Burden
ISBN : 0817637567
Genre : Science
File Size : 52.6 MB
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"This manual will give students a chance to explore the process and techniques of characterizing and purifying a protein and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis." "A summary of relevant techniques supplements actual laboratory exercises. Topics and techniques are chosen based on input from active researchers in the biotechnology/pharmaceutical sciences." "This book is designed for students of biotechnology and is suitable both as a course manual for academic and professional education programs, and for self-study and review. Tried and tested experiments from each area are assembled around a common theme. They not only teach valuable skills, but also demonstrate the research process used in biotechnology laboratories."--BOOK JACKET.Title Summary field provided by Blackwell North America, Inc. All Rights Reserved
Category: Science

Biotechnology For Waste Management And Site Restoration

Author : C. Ronneau
ISBN : 9789400914674
Genre : Nature
File Size : 32.21 MB
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Biotechnology for Waste Management and Site Restoration covers: waste management - solid, gaseous, liquid; site restoration - radioactivity, organics, toxic metals; educational, economic, social and business aspects; and international collaboration. International collaboration is growing apace and many concrete projects have been started. The body of knowledge is growing. Over the long term, it is envisaged that this international collaboration will result in a long-term scientific and technological strategy, new technologies and alternative solutions, and practical implementations of biotechnology for the nuclear and industrial sectors of the economy.
Category: Nature

Basic Laboratory Methods For Biotechnology

Author : Lisa A. Seidman
ISBN : 0137955359
Genre : Science
File Size : 44.45 MB
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A primary text for courses that focus on basic laboratory methods in biotechnology, and an ancillary text for any biology course that includes a laboratory component that want to focus on biotechnology. It provides an introduction to basic laboratory methods from the perspective of everyday work in the labs of biotechnology/bioscience industry.
Category: Science

Integrated Genomics

Author : Guy A. Caldwell
ISBN : 9780470095034
Genre : Science
File Size : 36.11 MB
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Integrated Genomics: A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols and a basic experimental design, this text offers experience with three different model systems. Students will become familiar with the simplicity and power of single-celled organisms, Escherichia coli and Saccharomyces cerevisiae, as they search for genes that interact and function within the nematode Caenorhabditis elegans. Incorporated throughout the course are exercises designed to offer students familiarity with the wealth of bioinformatics data that can be accessed on the World Wide Web. Following completion of interaction studies within the yeast, the course is designed to allow students to examine the functional consequences of reducing a gene’s function within the multicellular worm that is both simple and inexpensive to maintain within a laboratory. The inclusion of alternative experiments allow for flexibility in determining the ending date or goal of the laboratory, as well as working within the available budget and resources of most any classroom environment. Further striking features of this title are: An accompanying Web site providing PowerPoint slides, plus links to the internet, and regular updates as bioinformatics databases evolve and methods improve. Inclusion of modern genomic/proteomic technologies such as the yeast two-hybrid system and RNAi Detailed experimental protocols and easy access to instructional materials This discovery-based laboratory course provides excellent practical training for those pursuing career paths in biomedicine, pharmacy, and biotechnology.
Category: Science

Molecular Biology Techniques

Author : Sue Carson
ISBN : 9780123855442
Genre : Medical
File Size : 75.45 MB
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Category: Medical