BIOTECHNOLOGY A LABORATORY COURSE

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Biotechnology

Author : Jeffery M. Becker
ISBN : 9780323159135
Genre : Science
File Size : 78.45 MB
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Biotechnology: A Laboratory Course is a series of laboratory exercises demonstrating the in-depth experience and understanding of selected methods, techniques, and instrumentation used in biotechnology. This manual is an outgrowth of an introductory laboratory course for senior undergraduate and first year graduate students in the biological sciences at The University of Tennessee. This book is composed of 19 chapters and begins with some introductory notes on record keeping and safety rules. The first exercises include pH measurement, the use of micropipettors and spectrophotometers, the concept of aseptic technique, and preparation of culture media. The subsequent exercises involve the application of the growth curve, the isolation, purification, and concentration of plasmid DNA from Escherichia coli, and the process of agarose gel electrophoresis. Other exercises include the preparation, purification, and hybridization of probe, the transformation of Saccharomyces cerevisiae, the transformation of E. coli by plasmid DNA, and the principles and applications of protein assays. The final exercises explore the ?-galactosidase assay and the purification and determination of ?-galactosidase in permeabilized yeast cells. This book is of great value to undergraduate biotechnology and molecular biology students.
Category: Science

Biotechnology

Author : Jeffrey M. Becker
ISBN : 0120845628
Genre : Medical
File Size : 52.18 MB
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Asseptic technique and establishing pure cultures: the streak plate and culture transfer. Preparation of culture media. The growth curve. Isolation of plasmid DNA from escherichia coli: the mini-prep. Purification, concentration, and quantitation of DNA. Large-scale isolation of plasmid DNA by column chromatography. Amplification of a lacZ Gene fragment by the polymerase chain reaction. Restriction digestion and agarose gel electrophoresis. Southern transfer. Preparation, purification, and hybridization of probe. Transformation of saccharomyces cerevissiae. Isolation of plasmid from yeast and escherichia coli transformation. Protein assays. Qualitative assay for B-galactosidase in yeast colonies. Determination of B-galactosidase in permeabilized yeast cells. Assay of B-galactosidase in cell extracts. B-galactosidase purification. Western blot: probe of protein blot with antibody to B-galactosidase. Alternative protocols and experiments. Buffer solutions. Preparation of buffers and solutions. Properties of some common concentrated acids and bases. Use of micropipettors. List of cultures. Storage of cultures and DNA. Sterilization methods. Preparation of stock solutions for culture media. Growth in liquid medium. Determination of viable cells. Determination of cell mass. Determination of cell number. Nomenclature of strains. Glassware and plasticware. Preparation of tris and EDTA. Basic rules for handling enzymes. Effects of common contaminants on protein assays. Manufacturers' and distributors' addresses ...
Category: Medical

Biotechnology

Author : J. Kirk Brown
ISBN : 0983239606
Genre : Biotechnology
File Size : 67.65 MB
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Category: Biotechnology

Calculations For Molecular Biology And Biotechnology

Author : Frank H. Stephenson
ISBN : 012375691X
Genre : Science
File Size : 78.73 MB
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Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts
Category: Science

Dna Science

Author : David A. Micklos
ISBN : 0879696362
Genre : Science
File Size : 37.42 MB
Format : PDF
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. . .[A]n excellent molecular biology guide and lab manual for beginners. It is concocted uniquely as one part text that introduces the reader to the scientific concepts and one part well designed and tested laboratories in a friendly format.--"Science Books & Films."
Category: Science

Basic Laboratory Calculations For Biotechnology

Author : Lisa A. Seidman
ISBN : UCSD:31822034289918
Genre : Mathematics
File Size : 52.93 MB
Format : PDF
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To succeed in the lab, it is crucial to be comfortable with the math calculations that are part of everyday work. This accessible introduction to common laboratory techniques focuses on the basics, helping even readers with good math skills to practice the most frequently encountered types of problems. Discusses very common laboratory problems, all applied to real situations. Explores multiple strategies for solving problems for a better understanding of the underlying math. Includes hundreds of practice problems, all with solutions and many with boxed, complete explanations; plus hundreds of “story problems” relating to real situations in the lab. MARKET: A useful review for biotechnology laboratory professionals.
Category: Mathematics

Molecular Biology Techniques

Author : Sue Carson
ISBN : 9780123855442
Genre : Medical
File Size : 64.60 MB
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Category: Medical

Basic Laboratory Methods For Biotechnology

Author : Lisa A. Seidman
ISBN : 0137955359
Genre : Science
File Size : 78.2 MB
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A primary text for courses that focus on basic laboratory methods in biotechnology, and an ancillary text for any biology course that includes a laboratory component that want to focus on biotechnology. It provides an introduction to basic laboratory methods from the perspective of everyday work in the labs of biotechnology/bioscience industry.
Category: Science

Ana Techniqs In Biotechnology

Author : Goutam Bhowmik
ISBN : 0070700133
Genre : Biotechnology
File Size : 51.38 MB
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'Analytical Techniques in Biotechnology' by Bhowmik and Bose offers a unique compendium of fundamental experiments, which forms the crucial foundation to understand this contemporary subject that has enormous impact on many other branches of life sciences. In addition to its simple and lucid language, the main focus of the book is to equip a beginner with the skill and ability required to conduct independent experimentation and research in laboratories. Carefully structured to cover the complete spectra of subjects under the umbrella of 'Biotechnology', this book is sure to prove a ready reference for students, faculties and researchers, all alike!
Category: Biotechnology