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Author : Jeffrey M. Becker
ISBN : 0120845628
Genre : Medical
File Size : 79.88 MB
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Asseptic technique and establishing pure cultures: the streak plate and culture transfer. Preparation of culture media. The growth curve. Isolation of plasmid DNA from escherichia coli: the mini-prep. Purification, concentration, and quantitation of DNA. Large-scale isolation of plasmid DNA by column chromatography. Amplification of a lacZ Gene fragment by the polymerase chain reaction. Restriction digestion and agarose gel electrophoresis. Southern transfer. Preparation, purification, and hybridization of probe. Transformation of saccharomyces cerevissiae. Isolation of plasmid from yeast and escherichia coli transformation. Protein assays. Qualitative assay for B-galactosidase in yeast colonies. Determination of B-galactosidase in permeabilized yeast cells. Assay of B-galactosidase in cell extracts. B-galactosidase purification. Western blot: probe of protein blot with antibody to B-galactosidase. Alternative protocols and experiments. Buffer solutions. Preparation of buffers and solutions. Properties of some common concentrated acids and bases. Use of micropipettors. List of cultures. Storage of cultures and DNA. Sterilization methods. Preparation of stock solutions for culture media. Growth in liquid medium. Determination of viable cells. Determination of cell mass. Determination of cell number. Nomenclature of strains. Glassware and plasticware. Preparation of tris and EDTA. Basic rules for handling enzymes. Effects of common contaminants on protein assays. Manufacturers' and distributors' addresses ...
Category: Medical


Author : J. Kirk Brown
ISBN : 0983239649
Genre :
File Size : 31.46 MB
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Author : Jeffery M. Becker
ISBN : 9780323159135
Genre : Science
File Size : 50.6 MB
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Biotechnology: A Laboratory Course is a series of laboratory exercises demonstrating the in-depth experience and understanding of selected methods, techniques, and instrumentation used in biotechnology. This manual is an outgrowth of an introductory laboratory course for senior undergraduate and first year graduate students in the biological sciences at The University of Tennessee. This book is composed of 19 chapters and begins with some introductory notes on record keeping and safety rules. The first exercises include pH measurement, the use of micropipettors and spectrophotometers, the concept of aseptic technique, and preparation of culture media. The subsequent exercises involve the application of the growth curve, the isolation, purification, and concentration of plasmid DNA from Escherichia coli, and the process of agarose gel electrophoresis. Other exercises include the preparation, purification, and hybridization of probe, the transformation of Saccharomyces cerevisiae, the transformation of E. coli by plasmid DNA, and the principles and applications of protein assays. The final exercises explore the ?-galactosidase assay and the purification and determination of ?-galactosidase in permeabilized yeast cells. This book is of great value to undergraduate biotechnology and molecular biology students.
Category: Science

Molecular Biology Techniques

Author : Walt Ream
ISBN : 9780080536828
Genre : Science
File Size : 36.56 MB
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter
Category: Science

Calculations For Molecular Biology And Biotechnology

Author : Frank H. Stephenson
ISBN : 9780128025987
Genre : Computers
File Size : 68.86 MB
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Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins Includes more sample problems in every chapter for readers to practice concepts
Category: Computers

Basic Laboratory Calculations For Biotechnology

Author : Lisa A. Seidman
ISBN : UCSD:31822034289918
Genre : Mathematics
File Size : 54.57 MB
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To succeed in the lab, it is crucial to be comfortable with the math calculations that are part of everyday work. This accessible introduction to common laboratory techniques focuses on the basics, helping even readers with good math skills to practice the most frequently encountered types of problems. Discusses very common laboratory problems, all applied to real situations. Explores multiple strategies for solving problems for a better understanding of the underlying math. Includes hundreds of practice problems, all with solutions and many with boxed, complete explanations; plus hundreds of “story problems” relating to real situations in the lab. MARKET: A useful review for biotechnology laboratory professionals.
Category: Mathematics

Dna Science

Author : David A. Micklos
ISBN : 0879696362
Genre : Science
File Size : 48.6 MB
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. . .[A]n excellent molecular biology guide and lab manual for beginners. It is concocted uniquely as one part text that introduces the reader to the scientific concepts and one part well designed and tested laboratories in a friendly format.--"Science Books & Films."
Category: Science

Molecular Biology Techniques

Author : Heather Miller
ISBN : 9780123855459
Genre : Science
File Size : 79.27 MB
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Category: Science

Laboratory Manual For Genetic Engineering

ISBN : 9788120338142
Genre : Science
File Size : 30.6 MB
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This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering. The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology. KEY FEATURES : Includes about 60 different experiments. Contains several figures to reinforce the understanding of the techniques discussed. Gives useful information about preparation of stock solutions, DNA/protein conversions, restriction enzymes and their recognition sequences, and so on in Appendices.
Category: Science