A BEGINNERS GUIDE TO MICROARRAYS

Download A Beginners Guide To Microarrays ebook PDF or Read Online books in PDF, EPUB, and Mobi Format. Click Download or Read Online button to A BEGINNERS GUIDE TO MICROARRAYS book pdf for free now.

A Beginner S Guide To Microarrays

Author : Eric M. Blalock
ISBN : 9781441987600
Genre : Science
File Size : 21.94 MB
Format : PDF, ePub, Docs
Download : 219
Read : 277

A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including: -Cleaning and coating glass slides, -Designing oligonucleotide probes, -Constructing arrays for the detection and quantification of different bacterial species, -Preparing spotting solutions, -Troubleshooting spotting problems, -Setting up and running a core facility, -Normalizing background signal and controlling for systematic variance, -Designing experiments for maximum effect, -Analyzing data with statistical procedures, -Clustering data with machine-learning protocols.
Category: Science

Microarray Gene Expression Data Analysis

Author : Helen Causton
ISBN : 9781444311563
Genre : Science
File Size : 63.12 MB
Format : PDF, Kindle
Download : 824
Read : 1273

This guide covers aspects of designing microarray experiments and analysing the data generated, including information on some of the tools that are available from non-commercial sources. Concepts and principles underpinning gene expression analysis are emphasised and wherever possible, the mathematics has been simplified. The guide is intended for use by graduates and researchers in bioinformatics and the life sciences and is also suitable for statisticians who are interested in the approaches currently used to study gene expression. Microarrays are an automated way of carrying out thousands of experiments at once, and allows scientists to obtain huge amounts of information very quickly Short, concise text on this difficult topic area Clear illustrations throughout Written by well-known teachers in the subject Provides insight into how to analyse the data produced from microarrays
Category: Science

Microarrays

Author : Jang B. Rampal
ISBN : 9781588295897
Genre : Medical
File Size : 87.71 MB
Format : PDF
Download : 286
Read : 231

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.
Category: Medical

Protein Microarrays

Author : Mark Schena
ISBN : 0763731277
Genre : Medical
File Size : 28.71 MB
Format : PDF, Mobi
Download : 157
Read : 884

Ideal for allied health and pre-nursing students, Alcamos Fundamentals of Microbiology, Body Systems Edition, retains the engaging, student-friendly style and active learning approach for which award-winning author and educator Jeffrey Pommerville is known. It presents diseases, complete with new content on recent discoveries, in a manner that is directly applicable to students and organized by body system. A captivating art program, learning design format, and numerous case studies draw students into the text and make them eager to learn more about the fascinating world of microbiology.
Category: Medical

Microbial Functional Genomics

Author : Jizhong Zhou
ISBN : UOM:39015057781943
Genre : Medical
File Size : 75.86 MB
Format : PDF, ePub, Mobi
Download : 800
Read : 297

Genomics: toward a genome-level understanding of the structure, functions, and evolution of bioloical systems; Microbial diversity and genomics. Computational genome annotation. Microbial evolution from a genomics perspective. Computational methods for functional prediction of genes. DNA microarray technology. Microarray gene expression data analysis. Mutagenesis as a genomic tool for studying gene function. Mass spectrometry. Identification of protein-ligand interactions. The functional genomics of model organisms: addressing old questions from a new perspective. Functional genomic analysis of bacterial pathogens and environmentally significant microorganisms. The impact of genomics on antimicrobial drug discovery and toxicology. Application of microarray-based genomic technology to mutation analysis and microbial detection. Future perspectives: genomics beyond single cells.
Category: Medical

The German Language In The Digital Age

Author : Georg Rehm
ISBN : 9783642271663
Genre : Computers
File Size : 70.16 MB
Format : PDF, Docs
Download : 578
Read : 765

This white paper is part of a series that promotes knowledge about language technology and its potential. It addresses educators, journalists, politicians, language communities and others. The availability and use of language technology in Europe varies between languages. Consequently, the actions that are required to further support research and development of language technologies also differ for each language. The required actions depend on many factors, such as the complexity of a given language and the size of its community. META-NET, a Network of Excellence funded by the European Commission, has conducted an analysis of current language resources and technologies. This analysis focused on the 23 official European languages as well as other important national and regional languages in Europe. The results of this analysis suggest that there are many significant research gaps for each language. A more detailed expert analysis and assessment of the current situation will help maximise the impact of additional research and minimize any risks. META-NET consists of 54 research centres from 33 countries that are working with stakeholders from commercial businesses, government agencies, industry, research organisations, software companies, technology providers and European universities. Together, they are creating a common technology vision while developing a strategic research agenda that shows how language technology applications can address any research gaps by 2020.
Category: Computers

Introduction To Genomic Signal Processing With Control

Author : Aniruddha Datta
ISBN : 0849371988
Genre : Science
File Size : 21.25 MB
Format : PDF, Kindle
Download : 492
Read : 264

Studying large sets of genes and their collective function requires tools that can easily handle huge amounts of information. Recent research indicates that engineering approaches for prediction, signal processing, and control are well suited for studying multivariate interactions. A tutorial guide to the current engineering research in genomics, Introduction to Genomic Signal Processing with Control provides a state-of-the-art account of the use of control theory to obtain intervention strategies for gene regulatory networks. The book builds up the necessary molecular biology background with a basic review of organic chemistry and an introduction of DNA, RNA, and proteins, followed by a description of the processes of transcription and translation and the genetic code that is used to carry out the latter. It discusses control of gene expression, introduces genetic engineering tools such as microarrays and PCR, and covers cell cycle control and tissue renewal in multi-cellular organisms. The authors then delineate how the engineering approaches of classification and clustering are appropriate for carrying out gene-based disease classification. This leads naturally to expression prediction, which in turn leads to genetic regulatory networks. The book concludes with a discussion of control approaches that can be used to alter the behavior of such networks in the hope that this alteration will move the network from a diseased state to a disease-free state. Written by recognized leaders in this emerging field, the book provides the exact amount of molecular biology required to understand the engineering applications. It is a self-contained resource that spans the diverse disciplines of molecular biology and electrical engineering.
Category: Science

Leading Edge Environmental Biodegradation Research

Author : Lyman E. Pawley
ISBN : 1600219039
Genre : Science
File Size : 90.21 MB
Format : PDF
Download : 568
Read : 1333

This volume presents new leading-edge research on environmental biodegradation which is the destruction of organic compounds by micro-organisms. Micro-organisms, particularly bacteria, are responsible for the decomposition of both natural and synthetic organic compounds in nature. Mineralisation results in complete conversion of a compound to its inorganic mineral constituents (for example, carbon dioxide from carbon, sulphate or sulphide from organic sulfur, nitrate or ammonium from organic nitrogen, phosphate from organophosphates, or chloride from organochlorine). Since carbon comprises the greatest mass of organic compounds, mineralisation can be considered in terms of CO2 evolution. Radioactive carbon-14 (14C) isotopes enable scientists to distinguish between mineralisation arising from contaminants and soil organic matter. However, mineralisation of any compound is never 100% because some of it (10Chr(45)40% of the total amount degraded) is incorporated into the cell mass or products that become part of the amorphous soil organic matter, commonly referred to as humus. Thus, biodegradation comprises mineralisation and conversion to innocuous products, namely biomass and humus. Primary biodegradation is more limited in scope and refers to the disappearance of the compound as a result of its biotransformation to another product. Compounds that are readily biodegradable are generally utilised as growth substrates by single micro-organisms. Many of the components of petroleum products (and frequent ground-water contaminants), such as benzene, toluene, ethylbenzene, and xylene, are utilised by many genera of bacteria as sole carbon sources for growth and energy.
Category: Science