A BEGINNERS GUIDE TO MICROARRAYS

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A Beginner S Guide To Microarrays

Author : Eric M. Blalock
ISBN : 9781441987600
Genre : Science
File Size : 48.92 MB
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A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including: -Cleaning and coating glass slides, -Designing oligonucleotide probes, -Constructing arrays for the detection and quantification of different bacterial species, -Preparing spotting solutions, -Troubleshooting spotting problems, -Setting up and running a core facility, -Normalizing background signal and controlling for systematic variance, -Designing experiments for maximum effect, -Analyzing data with statistical procedures, -Clustering data with machine-learning protocols.
Category: Science

Microarray Gene Expression Data Analysis

Author : Helen Causton
ISBN : 9781444311563
Genre : Science
File Size : 43.13 MB
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This guide covers aspects of designing microarray experiments and analysing the data generated, including information on some of the tools that are available from non-commercial sources. Concepts and principles underpinning gene expression analysis are emphasised and wherever possible, the mathematics has been simplified. The guide is intended for use by graduates and researchers in bioinformatics and the life sciences and is also suitable for statisticians who are interested in the approaches currently used to study gene expression. Microarrays are an automated way of carrying out thousands of experiments at once, and allows scientists to obtain huge amounts of information very quickly Short, concise text on this difficult topic area Clear illustrations throughout Written by well-known teachers in the subject Provides insight into how to analyse the data produced from microarrays
Category: Science

Microbial Functional Genomics

Author : Jizhong Zhou
ISBN : 0471071900
Genre : Medical
File Size : 32.20 MB
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Genomics: toward a genome-level understanding of the structure, functions, and evolution of bioloical systems; Microbial diversity and genomics. Computational genome annotation. Microbial evolution from a genomics perspective. Computational methods for functional prediction of genes. DNA microarray technology. Microarray gene expression data analysis. Mutagenesis as a genomic tool for studying gene function. Mass spectrometry. Identification of protein-ligand interactions. The functional genomics of model organisms: addressing old questions from a new perspective. Functional genomic analysis of bacterial pathogens and environmentally significant microorganisms. The impact of genomics on antimicrobial drug discovery and toxicology. Application of microarray-based genomic technology to mutation analysis and microbial detection. Future perspectives: genomics beyond single cells.
Category: Medical

Bioinformatics Of Human Proteomics

Author : Xiangdong Wang
ISBN : 9789400758117
Genre : Science
File Size : 63.42 MB
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“Bioinformatics of Human Proteomics” discusses the development of methods, techniques and applications in the field of protein bioinformatics, an important direction in bioinformatics. It collects contributions from expert researchers in order to provide a practical guide to this complex field of study. The book covers the protein interaction network, drug discovery and development, the relationship between translational medicine and bioinformatics, and advances in proteomic methods, while also demonstrating important bioinformatics tools and methods available today for protein analysis, interpretation and predication. It is intended for experts or senior researchers in the fields of clinical research-related biostatistics, bioinformatics, computational biology, medicine, statistics, system biology, molecular diagnostics, biomarkers, or drug discovery and development. Dr.Xiangdong Wang works as a distinguished professor of Respiratory Medicine at Fudan University, Shanghai, China. He serves as Director of Biomedical Research Center, Fudan University Zhongshan Hospital and adjunct professor of Clinical Bioinformatics at Lund University, Sweden. His main research is focused on the role of clinical bioinformatics in the development of disease-specific biomarkers and dynamic network biomarkers, the molecular mechanism of organ dysfunction and potential therapies.
Category: Science

Microarrays

Author : Jang B. Rampal
ISBN : 9781588295897
Genre : Medical
File Size : 80.25 MB
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Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.
Category: Medical

Essentials Of Nucleic Acid Analysis

Author : Jacquie T. Keer
ISBN : 9780854043675
Genre : Science
File Size : 66.93 MB
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An indispensable handbook of the highest standard for those working in the fields of food analysis and forensic applications.
Category: Science

Leading Edge Environmental Biodegradation Research

Author : Lyman E. Pawley
ISBN : 1600219039
Genre : Science
File Size : 26.25 MB
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This volume presents new leading-edge research on environmental biodegradation which is the destruction of organic compounds by micro-organisms. Micro-organisms, particularly bacteria, are responsible for the decomposition of both natural and synthetic organic compounds in nature. Mineralisation results in complete conversion of a compound to its inorganic mineral constituents (for example, carbon dioxide from carbon, sulphate or sulphide from organic sulfur, nitrate or ammonium from organic nitrogen, phosphate from organophosphates, or chloride from organochlorine). Since carbon comprises the greatest mass of organic compounds, mineralisation can be considered in terms of CO2 evolution. Radioactive carbon-14 (14C) isotopes enable scientists to distinguish between mineralisation arising from contaminants and soil organic matter. However, mineralisation of any compound is never 100% because some of it (10Chr(45)40% of the total amount degraded) is incorporated into the cell mass or products that become part of the amorphous soil organic matter, commonly referred to as humus. Thus, biodegradation comprises mineralisation and conversion to innocuous products, namely biomass and humus. Primary biodegradation is more limited in scope and refers to the disappearance of the compound as a result of its biotransformation to another product. Compounds that are readily biodegradable are generally utilised as growth substrates by single micro-organisms. Many of the components of petroleum products (and frequent ground-water contaminants), such as benzene, toluene, ethylbenzene, and xylene, are utilised by many genera of bacteria as sole carbon sources for growth and energy.
Category: Science

Introduction To Genomic Signal Processing With Control

Author : Aniruddha Datta
ISBN : 0849371988
Genre : Science
File Size : 75.67 MB
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Studying large sets of genes and their collective function requires tools that can easily handle huge amounts of information. Recent research indicates that engineering approaches for prediction, signal processing, and control are well suited for studying multivariate interactions. A tutorial guide to the current engineering research in genomics, Introduction to Genomic Signal Processing with Control provides a state-of-the-art account of the use of control theory to obtain intervention strategies for gene regulatory networks. The book builds up the necessary molecular biology background with a basic review of organic chemistry and an introduction of DNA, RNA, and proteins, followed by a description of the processes of transcription and translation and the genetic code that is used to carry out the latter. It discusses control of gene expression, introduces genetic engineering tools such as microarrays and PCR, and covers cell cycle control and tissue renewal in multi-cellular organisms. The authors then delineate how the engineering approaches of classification and clustering are appropriate for carrying out gene-based disease classification. This leads naturally to expression prediction, which in turn leads to genetic regulatory networks. The book concludes with a discussion of control approaches that can be used to alter the behavior of such networks in the hope that this alteration will move the network from a diseased state to a disease-free state. Written by recognized leaders in this emerging field, the book provides the exact amount of molecular biology required to understand the engineering applications. It is a self-contained resource that spans the diverse disciplines of molecular biology and electrical engineering.
Category: Science

Practical Guide To High Risk Pregnancy And Delivery E Book

Author : Fernando Arias
ISBN : 9788131231630
Genre : Medical
File Size : 86.34 MB
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The third edition of the book, Practical Guide to High-Risk Pregnancy and Delivery, has been completely revised to present more up-to-date approach to the field. Several changes have been made in the book to keep up with multiple new developments and to facilitate the finding of information. However, the purpose of the book remains unchanged: to provide residents in Obstetrics and Gynecology, fellows in Maternal–Fetal Medicine, obstetricians, general physicians, and interested nurses and medical students with a source of practical information about complications of pregnancy. The book is organized into three sections: Fetal Medicine, Obstetrical Complications and Tropical Diseases in Pregnancy All chapters have been rearranged, expanded, and updated Several new flowcharts, tables, and boxes have been included to make the coverage more comprehensive and contemporary Important points at the end of every chapter help in quick recapitulation. Separate chapters on Hypertensive Disorders in Pregnancy, Diabetes and Pregnancy, and Cardiac Diseases in Pregnancy.
Category: Medical